Enhanced Ultrasound-assisted Enzymatic Hydrolysis Extraction of Quinolizidine Alkaloids from Sophora Alopecuroides L. Seeds

Overview

Journal J Nat Med

Publisher Springer

Date 2017 Dec 14

PMID 29236225

Citations 8

Authors

Hanqing Wang

Yue Tong

Wei Li

Xia Zhang

Xiaojuan Gao

Jingjiao Yong

Jianjun Zhao

Kazuo Koike

Affiliations

Soon will be listed here.

Abstract

Quinolizidine alkaloids are the main bioactive components in Sophora alopecuroides L. This study reports a novel ultrasound-assisted enzymatic hydrolysis method for the extraction of these important alkaloids. Box-Behnken design, a widely used response surface methodology, was used to investigate the effects of process variables on ultrasound bath-assisted enzymatic hydrolysis (UAEH) extraction. Four independent variables, pH, extraction temperature (°C), extraction time (min) and solvent-to-material ratio (mL/g), were studied. For the extraction of sophocarpine, oxysophocarpine, oxymatrine, matrine, sophoramine, sophoridine and cytisine, the optimal UAEH condition was found to be a pH of 5, extraction temperature of 54 °C, extraction time of 60 min and solvent-to-material ratio of 112 mL/g. The experimental values obtained under optimal conditions were fairly consistent with the predicted values. UAEH extraction was then compared with reflux heating, enzymatic extraction and ultrasound-assisted extraction. Of these extraction methods, UAEH extraction under optimal conditions produced the highest yield for seven types of alkaloids. In addition, UAEH extraction resulted in lower ingredient degradation than reflux heating extraction.

Citing Articles

Ultrafast and Highly Efficient Laser Extraction of Matrine and Oxymatrine from for the Anticancer Activity.

Wu P, Wang P, Cao W, Liu N, Zou H, Yang G ACS Omega. 2024; 9(37):38846-38854.

PMID: 39310152 PMC: 11411678. DOI: 10.1021/acsomega.4c05003.

Community ecological succession of endophytic fungi associates with medicinal compound accumulation in .

Ju M, Zhang Q, Wang R, Yan S, Zhang Q, Li P Microbiol Spectr. 2024; 12(2):e0307623.

PMID: 38236025 PMC: 10845968. DOI: 10.1128/spectrum.03076-23.

Recent advancement in ultrasound-assisted novel technologies for the extraction of bioactive compounds from herbal plants: a review.

Islam M, Malakar S, Rao M, Kumar N, Sahu J Food Sci Biotechnol. 2023; 32(13):1763-1782.

PMID: 37781053 PMC: 10541372. DOI: 10.1007/s10068-023-01346-6.

Correlation in endophytic fungi community diversity and bioactive compounds of .

Ju M, Zhang Q, Wang R, Yan S, Li Z, Li P Front Microbiol. 2022; 13:955647.

PMID: 36118208 PMC: 9475766. DOI: 10.3389/fmicb.2022.955647.

Small molecules of herbal origin for osteoarthritis treatment: in vitro and in vivo evidence.

Zhang P, Li K, Kamali A, Ziadlou R, Ahmad P, Wang X Arthritis Res Ther. 2022; 24(1):105.

PMID: 35545776 PMC: 9092710. DOI: 10.1186/s13075-022-02785-y.

References

Yifeng M, Bin W, Weiqiao Z, Yongming Q, Bing L, Xiaojie L . Neuroprotective effect of sophocarpine against transient focal cerebral ischemia via down-regulation of the acid-sensing ion channel 1 in rats. Brain Res. 2011; 1382:245-51. DOI: 10.1016/j.brainres.2011.01.004. View

Liu H, Sun Y, Gao Y, Chen F, Xu M, Liu Z . The analgesic effect and mechanism of the combination of sodium ferulate and oxymatrine. Neurochem Res. 2010; 35(9):1368-75. DOI: 10.1007/s11064-010-0193-4. View

Chen X, Marshall W . Enzymatic digestion-high-pressure homogenization prior to slurry introduction graphite furnace atomic absorption spectrometry for selenium determination in plant and animal tissues. J Agric Food Chem. 1999; 47(9):3727-32. DOI: 10.1021/jf981218w. View

Jiang H, Hou C, Zhang S, Xie H, Zhou W, Jin Q . Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells. Eur J Pharmacol. 2007; 559(2-3):98-108. DOI: 10.1016/j.ejphar.2006.12.017. View

Zhang S, Qi J, Sun L, Cheng B, Pan S, Zhou M . Matrine induces programmed cell death and regulates expression of relevant genes based on PCR array analysis in C6 glioma cells. Mol Biol Rep. 2008; 36(4):791-9. DOI: 10.1007/s11033-008-9247-y. View

Konwarh R, Pramanik S, Kalita D, Mahanta C, Karak N . Ultrasonication--a complementary 'green chemistry' tool to biocatalysis: a laboratory-scale study of lycopene extraction. Ultrason Sonochem. 2011; 19(2):292-9. DOI: 10.1016/j.ultsonch.2011.07.010. View

Wang H, Guo S, Qian D, Qian Y, Duan J . Comparative analysis of quinolizidine alkaloids from different parts of Sophora alopecuroides seeds by UPLC-MS/MS. J Pharm Biomed Anal. 2012; 67-68:16-21. DOI: 10.1016/j.jpba.2012.04.024. View

Teng H, Choi Y . Optimization of ultrasonic-assisted extraction of bioactive alkaloid compounds from rhizoma coptidis (Coptis chinensis Franch.) using response surface methodology. Food Chem. 2013; 142:299-305. DOI: 10.1016/j.foodchem.2013.06.136. View

Yuan Z, Xiao X, Li G . Dynamic pH junction high-speed counter-current chromatography coupled with microwave-assisted extraction for online separation and purification of alkaloids from Stephania cepharantha. J Chromatogr A. 2013; 1317:203-10. DOI: 10.1016/j.chroma.2013.07.063. View

10.

Li X, Zhou R, Zheng P, Yan L, Wu Y, Xiao X . Cardioprotective effect of matrine on isoproterenol-induced cardiotoxicity in rats. J Pharm Pharmacol. 2010; 62(4):514-20. DOI: 10.1211/jpp.62.04.0015. View

11.

Wang Y, Zhao W, Xue R, Zhou Z, Liu F, Han Y . Oxymatrine inhibits hepatitis B infection with an advantage of overcoming drug-resistance. Antiviral Res. 2011; 89(3):227-31. DOI: 10.1016/j.antiviral.2011.01.005. View

12.

HELMAN J . Effects of some enzymes, surface-active agents, and calcium chloride on the aqueous extraction of alkaloids from belladonna leaves. J Pharm Sci. 1969; 58(9):1085-8. DOI: 10.1002/jps.2600580909. View

13.

Miguez-Framil M, Moreda-Pineiro A, Bermejo-Barrera P, Lopez P, Tabernero M, Bermejo A . Improvements on enzymatic hydrolysis of human hair for illicit drug determination by gas chromatography/mass spectrometry. Anal Chem. 2007; 79(22):8564-70. DOI: 10.1021/ac071337n. View