A Novel Type I Cystatin of Parasite Origin with Atypical Legumain-binding Domain

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 14

PMID 29235483

Citations 11

Authors

Jana Ilgova

Lucie Jedlickova

Hana Dvorakova

Michal Benovics

Libor Mikes

Lubomir Janda

Jiri Vorel

Pavel Roudnicky

David Potesil

Zbynek Zdrahal

Milan Gelnar

Martin Kasny

Affiliations

Soon will be listed here.

Abstract

Parasite inhibitors of cysteine peptidases are known to influence a vast range of processes linked to a degradation of either the parasites' own proteins or proteins native to their hosts. We characterise a novel type I cystatin (stefin) found in a sanguinivorous fish parasite Eudiplozoon nipponicum (Platyhelminthes: Monogenea). We have identified a transcript of its coding gene in the transcriptome of adult worms. Its amino acid sequence is similar to other stefins except for containing a legumain-binding domain, which is in this type of cystatins rather unusual. As expected, the recombinant form of E. nipponicum stefin (rEnStef) produced in Escherichia coli inhibits clan CA peptidases - cathepsins L and B of the worm - via the standard papain-binding domain. It also blocks haemoglobinolysis by cysteine peptidases in the worm's excretory-secretory products and soluble extracts. Furthermore, we had confirmed its ability to inhibit clan CD asparaginyl endopeptidase (legumain). The presence of a native EnStef in the excretory-secretory products of adult worms, detected by mass spectrometry, suggests that this protein has an important biological function at the host-parasite interface. We discuss the inhibitor's possible role in the regulation of blood digestion, modulation of antigen presentation, and in the regeneration of host tissues.

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