The Potential Coordination of the Heat-Shock Proteins and Antioxidant Enzyme Genes of in Response to Thermal Stress

Overview

Journal Front Physiol

Date 2017 Dec 14

PMID 29234290

Citations 16

Authors

Zhi-Wei Kang

Fang-Hua Liu

Xiang Liu

Wen-Bo Yu

Xiao-Ling Tan

Shi-Ze Zhang

Hong-Gang Tian

Tong-Xian Liu

Affiliations

Soon will be listed here.

Abstract

is one of the most important aphid natural enemies and has been successfully used to control and other aphid species. High temperature in summer is one of the key barriers for the application of in the field and greenhouse. In this work, we investigated the biological performance of and the response of heat-shock proteins and antioxidant enzymes under high temperature. The results showed that could not survive at 40°C and female exhibited a higher survival in 35°C. Furthermore, the short term exposure to high temperature negatively affected the performance of especially parasitism efficiency. Under short-term heating, the expression of and showed an increased trend, whereas initially increased and then decreased. In 35°C, the expressions of and in female were higher than those in male, whereas the expression of exhibited an opposite trend. Besides the HSPs, we also quantified the expression levels of 11 antioxidant enzyme genes: and . We found that the sex-specific expression of and were highly consistent with sex-specific heat shock survival rates at 35°C. Furthermore, when the temperature was above 30°C, the activities of GST, SOD, CAT and POD were significantly increased; however, there was no significant difference of the CAT activity between the male and female at 35°C. Collectively, all of these results suggested that the protection of thermal damage is coordinated by HSPs and antioxidant enzymes in . Based on the heat tolerance abilities of many aphid natural enemies, we also discussed an integrated application strategy of many aphid enemies in summer.

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References

Wang S, Liang N, Tang R, Liu Y, Liu T . Brief Heat Stress Negatively Affects the Population Fitness and Host Feeding of Aphelinus asychis (Hymenoptera: Aphelinidae) Parasitizing Myzus persicae (Hemiptera: Aphididae). Environ Entomol. 2016; 45(3):719-725. DOI: 10.1093/ee/nvw016. View

Nguyen T, Bressac C, Chevrier C . Heat stress affects male reproduction in a parasitoid wasp. J Insect Physiol. 2012; 59(3):248-54. DOI: 10.1016/j.jinsphys.2012.12.001. View

Yang L, Huang H, Wang J . Antioxidant responses of citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae), exposed to thermal stress. J Insect Physiol. 2010; 56(12):1871-6. DOI: 10.1016/j.jinsphys.2010.08.006. View

Li Y, Zhao Q, Duan X, Song C, Chen M . Transcription of four Rhopalosiphum padi (L.) heat shock protein genes and their responses to heat stress and insecticide exposure. Comp Biochem Physiol A Mol Integr Physiol. 2016; 205:48-57. DOI: 10.1016/j.cbpa.2016.12.021. View

Luo S, Ahola V, Shu C, Xu C, Wang R . Heat shock protein 70 gene family in the Glanville fritillary butterfly and their response to thermal stress. Gene. 2014; 556(2):132-41. DOI: 10.1016/j.gene.2014.11.043. View

Feng Y, Liao C, Xia W, Jiang X, Shang F, Yuan G . Regulation of three isoforms of SOD gene by environmental stresses in citrus red mite, Panonychus citri. Exp Appl Acarol. 2015; 67(1):49-63. DOI: 10.1007/s10493-015-9930-3. View

Qiu X, Shao Y, Miao S, Wang L . The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones. Cell Mol Life Sci. 2006; 63(22):2560-70. PMC: 11136209. DOI: 10.1007/s00018-006-6192-6. View

Knorr E, Vilcinskas A . Post-embryonic functions of HSP90 in Tribolium castaneum include the regulation of compound eye development. Dev Genes Evol. 2011; 221(5-6):357-62. DOI: 10.1007/s00427-011-0379-z. View

Lu K, Chen X, Liu W, Zhou Q . Characterization of heat shock cognate protein 70 gene and its differential expression in response to thermal stress between two wing morphs of Nilaparvata lugens (Stål). Comp Biochem Physiol A Mol Integr Physiol. 2016; 199:47-53. DOI: 10.1016/j.cbpa.2016.05.009. View

10.

Qin G, Jia M, Liu T, Zhang X, Guo Y, Zhu K . Characterization and functional analysis of four glutathione S-transferases from the migratory locust, Locusta migratoria. PLoS One. 2013; 8(3):e58410. PMC: 3591310. DOI: 10.1371/journal.pone.0058410. View

11.

Rajpurohit S, Hanus R, Vrkoslav V, Behrman E, Bergland A, Petrov D . Adaptive dynamics of cuticular hydrocarbons in Drosophila. J Evol Biol. 2016; 30(1):66-80. PMC: 5214518. DOI: 10.1111/jeb.12988. View

12.

Picard D . Heat-shock protein 90, a chaperone for folding and regulation. Cell Mol Life Sci. 2002; 59(10):1640-8. PMC: 11337538. DOI: 10.1007/pl00012491. View

13.

Folk D, Zwollo P, Rand D, Gilchrist G . Selection on knockdown performance in Drosophila melanogaster impacts thermotolerance and heat-shock response differently in females and males. J Exp Biol. 2006; 209(Pt 20):3964-73. DOI: 10.1242/jeb.02463. View

14.

Sun Y, Sheng Y, Bai L, Zhang Y, Xiao Y, Xiao L . Characterizing heat shock protein 90 gene of Apolygus lucorum (Meyer-Dür) and its expression in response to different temperature and pesticide stresses. Cell Stress Chaperones. 2014; 19(5):725-39. PMC: 4147072. DOI: 10.1007/s12192-014-0500-0. View

15.

Kim K, Kim R, Kim S . Crystal structure of a small heat-shock protein. Nature. 1998; 394(6693):595-9. DOI: 10.1038/29106. View

16.

Will T, Schmidtberg H, Skaljac M, Vilcinskas A . Heat shock protein 83 plays pleiotropic roles in embryogenesis, longevity, and fecundity of the pea aphid Acyrthosiphon pisum. Dev Genes Evol. 2016; 227(1):1-9. PMC: 5203865. DOI: 10.1007/s00427-016-0564-1. View

17.

Ali A, Rashid M, Huang Q, Wong C, Lei C . Response of antioxidant enzymes in Mythimna separata (Lepidoptera: Noctuidae) exposed to thermal stress. Bull Entomol Res. 2016; 107(3):382-390. DOI: 10.1017/S0007485316001000. View

18.

Wang Y, Wang L, Zhu Z, Ma W, Lei C . The molecular characterization of antioxidant enzyme genes in Helicoverpa armigera adults and their involvement in response to ultraviolet-A stress. J Insect Physiol. 2012; 58(9):1250-8. DOI: 10.1016/j.jinsphys.2012.06.012. View

19.

Lu M, Hua J, Cui Y, Du Y . Five small heat shock protein genes from Chilo suppressalis: characteristics of gene, genomic organization, structural analysis, and transcription profiles. Cell Stress Chaperones. 2013; 19(1):91-104. PMC: 3857428. DOI: 10.1007/s12192-013-0437-8. View

20.

Krebs R, Thompson K . Direct and correlated effects of selection on flight after exposure to thermal stress in Drosophila melanogaster. Genetica. 2006; 128(1-3):217-25. DOI: 10.1007/s10709-005-5704-x. View