Re-engineering the Two-component Systems As Light-regulated in

Overview

Journal J Biosci

Specialties Biochemistry
Biology

Date 2017 Dec 13

PMID 29229875

Citations 4

Authors

Siya Ma

Siwei Luo

L I Wu

Zhi Liang

Jia-Rui Wu

Affiliations

Soon will be listed here.

Abstract

Bacteria live in environments with dynamic changes. To sense and respond to different external stimuli, bacteria make use of various sensor-response circuits, called two-component systems (TCSs). A TCS comprises a histidine protein kinase (HK) sensing environmental stimuli and a response regulator protein (RR) regulating downstream genes. The two components are coupled via a phosphorylation control mechanism. In a recent study, we adopted an optogenetics approach to re-engineer the sensor HKs in as a light-sensing fusion protein. We constructed a light-controllable HK by replacing the original signal-specific sensing domain of HK with the light-sensing domain of Cph1 from , so that HK can be investigated by red light. Here, we extended the study to other 16 HK-RR TCSs and constructed a library of light-responsible HK-Cph1 chimeras. By taking the NarX-NarL system as an example, we demonstrated the light responsiveness of the constructed chimera and investigated the frequency response of the NarXNarL system. The constructed library serves as a toolkit for future TCS study using optogenetics approach.

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References

Deisseroth K . Optogenetics. Nat Methods. 2010; 8(1):26-9. PMC: 6814250. DOI: 10.1038/nmeth.f.324. View

Hersen P, McClean M, Mahadevan L, Ramanathan S . Signal processing by the HOG MAP kinase pathway. Proc Natl Acad Sci U S A. 2008; 105(20):7165-70. PMC: 2386076. DOI: 10.1073/pnas.0710770105. View

Domian I, Quon K, Shapiro L . The control of temporal and spatial organization during the Caulobacter cell cycle. Curr Opin Genet Dev. 1996; 6(5):538-44. DOI: 10.1016/s0959-437x(96)80081-5. View

Hanahan D . Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983; 166(4):557-80. DOI: 10.1016/s0022-2836(83)80284-8. View

Essen L, Mailliet J, Hughes J . The structure of a complete phytochrome sensory module in the Pr ground state. Proc Natl Acad Sci U S A. 2008; 105(38):14709-14. PMC: 2567182. DOI: 10.1073/pnas.0806477105. View

Falke J, Bass R, Butler S, Chervitz S, Danielson M . The two-component signaling pathway of bacterial chemotaxis: a molecular view of signal transduction by receptors, kinases, and adaptation enzymes. Annu Rev Cell Dev Biol. 1997; 13:457-512. PMC: 2899694. DOI: 10.1146/annurev.cellbio.13.1.457. View

Aravind L, Ponting C . The cytoplasmic helical linker domain of receptor histidine kinase and methyl-accepting proteins is common to many prokaryotic signalling proteins. FEMS Microbiol Lett. 1999; 176(1):111-6. DOI: 10.1111/j.1574-6968.1999.tb13650.x. View

Davis S, Vener A, Vierstra R . Bacteriophytochromes: phytochrome-like photoreceptors from nonphotosynthetic eubacteria. Science. 2000; 286(5449):2517-20. DOI: 10.1126/science.286.5449.2517. View

Moir J, Wood N . Nitrate and nitrite transport in bacteria. Cell Mol Life Sci. 2001; 58(2):215-24. PMC: 11146482. DOI: 10.1007/PL00000849. View

10.

Baek J, Lee S . Transcriptome analysis of phosphate starvation response in Escherichia coli. J Microbiol Biotechnol. 2007; 17(2):244-52. View

11.

Wu J, Newton A . Regulation of the Caulobacter flagellar gene hierarchy; not just for motility. Mol Microbiol. 1997; 24(2):233-9. DOI: 10.1046/j.1365-2958.1997.3281691.x. View

12.

Perego M . Kinase-phosphatase competition regulates Bacillus subtilis development. Trends Microbiol. 1998; 6(9):366-70. DOI: 10.1016/s0966-842x(98)01350-x. View

13.

Olson E, Tabor J . Optogenetic characterization methods overcome key challenges in synthetic and systems biology. Nat Chem Biol. 2014; 10(7):502-11. DOI: 10.1038/nchembio.1559. View

14.

Makino K, Shinagawa H, Amemura M, Kawamoto T, Yamada M, Nakata A . Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins. J Mol Biol. 1989; 210(3):551-9. DOI: 10.1016/0022-2836(89)90131-9. View

15.

Stewart V . Biochemical Society Special Lecture. Nitrate- and nitrite-responsive sensors NarX and NarQ of proteobacteria. Biochem Soc Trans. 2003; 31(Pt 1):1-10. DOI: 10.1042/bst0310001. View

16.

Schmidl S, Sheth R, Wu A, Tabor J . Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol. 2014; 3(11):820-31. DOI: 10.1021/sb500273n. View

17.

Alon U, Surette M, Barkai N, Leibler S . Robustness in bacterial chemotaxis. Nature. 1999; 397(6715):168-71. DOI: 10.1038/16483. View

18.

Williams S, Stewart V . Functional similarities among two-component sensors and methyl-accepting chemotaxis proteins suggest a role for linker region amphipathic helices in transmembrane signal transduction. Mol Microbiol. 1999; 33(6):1093-102. DOI: 10.1046/j.1365-2958.1999.01562.x. View

19.

Bourret R, Borkovich K, Simon M . Signal transduction pathways involving protein phosphorylation in prokaryotes. Annu Rev Biochem. 1991; 60:401-41. DOI: 10.1146/annurev.bi.60.070191.002153. View

20.

Toettcher J, Weiner O, Lim W . Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell. 2013; 155(6):1422-34. PMC: 3925772. DOI: 10.1016/j.cell.2013.11.004. View