The Dynamics of Native Atoh7 Protein Expression During Mouse Retinal Histogenesis, Revealed with a New Antibody

Overview

Journal Gene Expr Patterns

Publisher Elsevier

Date 2017 Dec 12

PMID 29225067

Citations 19

Authors

Joel B Miesfeld

Tom Glaser

Nadean L Brown

Affiliations

Soon will be listed here.

Abstract

The Atoh7 transcription factor catalyzes the rate-limiting step in the specification of retinal ganglion cells (RGCs). As a tool to study vertebrate retinal development, we validate an antibody that recognizes human and mouse Atoh7 polypeptide, using informative knockout and transgenic mouse tissues and overexpression experiments. The transient features of Atoh7 protein expression during retinal neurogenesis match the expected pattern at the tissue and cellular level. Further, we compare endogenous Atoh7 to established RGC markers, reporter mouse lines and cell cycle markers, demonstrating the utility of the antibody to investigate molecular mechanisms of retinal histogenesis.

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