Tension Force-induced Bone Formation in Orthodontic Tooth Movement Via Modulation of the GSK-3β/β-catenin Signaling Pathway

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2017 Dec 11

PMID 29224185

Citations 7

Authors

Yelin Mao

Liangliang Wang

Ye Zhu

Yu Liu

Hongwei Dai

Jianping Zhou

Dechun Geng

Lin Wang

Yong Ji

Affiliations

Soon will be listed here.

Abstract

Orthodontic force-induced osteogenic differentiation and bone formation at tension sites play a critical role in orthodontic tooth movement. However, the molecular mechanism underlying this phenomenon is poorly understood. In the current study, we investigated the involvement of the GSK-3β/β-catenin signaling pathway, which is critical for bone formation during tooth movement. We established a rat tooth movement model to test the hypothesis that orthodontic force may stimulate bone formation at the tension site of the moved tooth and promote the rate of tooth movement via regulation of the GSK-3β/β-catenin signaling pathway. Our results showed that continued mechanical loading increased the distance between the first and second molar in rats. In addition, the loading force increased bone formation at the tension site, and also increased phospho-Ser9-GSK-3β expression and β-catenin signaling pathway activity. Downregulation of GSK-3β activity further increased bone parameters, including bone mineral density, bone volume to tissue volume and trabecular thickness, as well as ALP- and osterix-positive cells at tension sites during tooth movement. However, ICG-001, the β-catenin selective inhibitor, reversed the positive effects of GSK-3β inhibition. In addition, pharmaceutical inhibition of GSK-3β or local treatment with β-catenin inhibitor did not influence the rate of tooth movement. Based on these results, we concluded that GSK-3β/β-catenin signaling contributes to the bone remodeling induced by orthodontic forces, and can be used as a potential therapeutic target in clinical dentistry.

Citing Articles

Investigation of Oxidative-Stress Impact on Human Osteoblasts During Orthodontic Tooth Movement Using an In Vitro Tension Model.

Hosseini S, Diegelmann J, Folwaczny M, Sabbagh H, Otto S, Kakoschke T Int J Mol Sci. 2025; 25(24.

PMID: 39769290 PMC: 11677893. DOI: 10.3390/ijms252413525.

Micro-computed tomography evaluation of the effects of orthodontic force on immature maxillary first molars and alveolar bone mineral density of Sprague-Dawley rats.

Wang J, Zhang R, Zhang Z, Geng C, Zhang Y Korean J Orthod. 2023; 53(3):205-216.

PMID: 37226513 PMC: 10212771. DOI: 10.4041/kjod22.209.

Compressive force regulates GSK-3β in osteoclasts contributing to alveolar bone resorption during orthodontic tooth movement in vivo.

Liu Y, Wu K, Cui X, Mao Y Heliyon. 2022; 8(8):e10379.

PMID: 36061014 PMC: 9433691. DOI: 10.1016/j.heliyon.2022.e10379.

Vertebral Body Tethering in 49 Adolescent Patients after Peak Height Velocity for the Treatment of Idiopathic Scoliosis: 2-5 Year Follow-Up.

Meyers J, Eaker L, Zhang J, Di Pauli von Treuheim T, Lonner B J Clin Med. 2022; 11(11).

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Mechanical stimuli-mediated modulation of bone cell function-implications for bone remodeling and angiogenesis.

Liang W, Wu X, Dong Y, Chen X, Zhou P, Xu F Cell Tissue Res. 2021; 386(3):445-454.

PMID: 34665321 DOI: 10.1007/s00441-021-03532-6.

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