Apolipoprotein E Polymorphism and the Risk of Aneurysmal Subarachnoid Hemorrhage in a South Indian Population

Overview

Journal Cell Mol Biol Lett

Publisher Biomed Central

Specialties Biochemistry
Cell Biology
Molecular Biology

Date 2017 Dec 8

PMID 29213291

Authors

Arati Suvatha

Sibin Madathan Kandi

Dhananjaya Ishwara Bhat

Narasinga Rao

Vikas Vazhayil

Chetan Ghati Kasturirangan

Affiliations

Soon will be listed here.

Abstract

Background: The rupture of a brain aneurysm causes bleeding in the subarachnoid space. This is known as aneurysmal subarachnoid hemorrhage (aSAH). We evaluated the association of apolipoprotein E () polymorphism and the risk of aSAH in a South Indian population.

Methods: The study was performed on 200 subjects with aSAH and 253 healthy control subjects. Blood samples (5 ml) were used to isolate DNA and genotyping was performed for rs7412 and rs429358 using a Taqman allelic discrimination assay. Statistical software R.3.0.11 was used to statistically analyze the data and a value < 0.05 was considered as statistically significant.

Results: We found a significant association with the risk of aSAH in ε3/ ε4 genetic model (OR = 1.91, 95% CI = 1.16-3.14, = 0.01). However, in the other genetic models and allele frequency, there was no significant association with the risk of aSAH. In subtyping, we found a significant association of ε2 allele frequency with posterior communicating artery (PCOM) aneurysm (OR = 3.59, 95% CI = 1.11-11.64, = 0.03).

Conclusion: Our results suggest that polymorphism has an influence on the risk of aSAH in this South Indian population, specifically in the PCOM subtype.

References

McCarron M, Nicoll J . High frequency of apolipoprotein E epsilon 2 allele is specific for patients with cerebral amyloid angiopathy-related haemorrhage. Neurosci Lett. 1998; 247(1):45-8. DOI: 10.1016/s0304-3940(98)00286-9. View

DSouza S . Aneurysmal Subarachnoid Hemorrhage. J Neurosurg Anesthesiol. 2014; 27(3):222-40. PMC: 4463029. DOI: 10.1097/ANA.0000000000000130. View

Stober T, Sen S, Anstatt T, Freier G, Schimrigk K . Direct evidence of hypertension and the possible role of post-menopause oestrogen deficiency in the pathogenesis of berry aneurysms. J Neurol. 1985; 232(2):67-72. DOI: 10.1007/BF00313903. View

Achouri-Rassas A, Ben Ali N, Cherif A, Fray S, Siala H, Zakraoui N . Association between ACE polymorphism, cognitive phenotype and APOE E4 allele in a Tunisian population with Alzheimer disease. J Neural Transm (Vienna). 2015; 123(3):317-21. DOI: 10.1007/s00702-015-1468-3. View

Lanterna L, Rigoldi M, Tredici G, Biroli F, Cesana C, Gaini S . APOE influences vasospasm and cognition of noncomatose patients with subarachnoid hemorrhage. Neurology. 2005; 64(7):1238-44. DOI: 10.1212/01.WNL.0000156523.77347.B4. View

Enzinger C, Ropele S, Smith S, Strasser-Fuchs S, Poltrum B, Schmidt H . Accelerated evolution of brain atrophy and "black holes" in MS patients with APOE-epsilon 4. Ann Neurol. 2004; 55(4):563-9. DOI: 10.1002/ana.20027. View

Nabulsi A, Folsom A, White A, Patsch W, Heiss G, Wu K . Association of hormone-replacement therapy with various cardiovascular risk factors in postmenopausal women. The Atherosclerosis Risk in Communities Study Investigators. N Engl J Med. 1993; 328(15):1069-75. DOI: 10.1056/NEJM199304153281501. View

Kapoor K, Kak V . Incidence of intracranial aneurysms in north-west Indian population. Neurol India. 2003; 51(1):22-6. View

Steinmetz A, Jakobs C, Motzny S, KAFFARNIK H . Differential distribution of apolipoprotein E isoforms in human plasma lipoproteins. Arteriosclerosis. 1989; 9(3):405-11. DOI: 10.1161/01.atv.9.3.405. View

10.

Singh P, Singh M, Mastana S . APOE distribution in world populations with new data from India and the UK. Ann Hum Biol. 2006; 33(3):279-308. DOI: 10.1080/03014460600594513. View

11.

Teasdale G, Murray G, Nicoll J . The association between APOE epsilon4, age and outcome after head injury: a prospective cohort study. Brain. 2005; 128(Pt 11):2556-61. DOI: 10.1093/brain/awh595. View

12.

Zheng L, Duan J, Duan X, Zhou W, Chen C, Li Y . Association of Apolipoprotein E (ApoE) Polymorphism with Alzheimer's Disease in Chinese Population. Curr Alzheimer Res. 2016; 13(8):912-7. DOI: 10.2174/1567205013666160401115307. View

13.

Mhurchu C, Anderson C, Jamrozik K, Hankey G, Dunbabin D . Hormonal factors and risk of aneurysmal subarachnoid hemorrhage: an international population-based, case-control study. Stroke. 2001; 32(3):606-12. DOI: 10.1161/01.str.32.3.606. View

14.

Mineharu Y, Inoue K, Inoue S, Yamada S, Nozaki K, Takenaka K . Association analysis of common variants of ELN, NOS2A, APOE and ACE2 to intracranial aneurysm. Stroke. 2006; 37(5):1189-94. DOI: 10.1161/01.STR.0000217408.91389.4d. View

15.

Wang Q, Wang W, Wu L, Liu L, Han L . Meta-analysis of APOE ε2/ε3/ε4 polymorphism and cerebral infarction. J Neural Transm (Vienna). 2013; 120(10):1479-89. DOI: 10.1007/s00702-013-1019-8. View

16.

Bellosta S, Nathan B, Orth M, Dong L, Mahley R, Pitas R . Stable expression and secretion of apolipoproteins E3 and E4 in mouse neuroblastoma cells produces differential effects on neurite outgrowth. J Biol Chem. 1995; 270(45):27063-71. DOI: 10.1074/jbc.270.45.27063. View

17.

Henderson V . Cognitive changes after menopause: influence of estrogen. Clin Obstet Gynecol. 2008; 51(3):618-26. PMC: 2637911. DOI: 10.1097/GRF.0b013e318180ba10. View

18.

Davignon J, GREGG R, Sing C . Apolipoprotein E polymorphism and atherosclerosis. Arteriosclerosis. 1988; 8(1):1-21. DOI: 10.1161/01.atv.8.1.1. View

19.

Ziemba-Davis M, Bohnstedt B, Payner T, Leipzig T, Palmer E, Cohen-Gadol A . Incidence, epidemiology, and treatment of aneurysmal subarachnoid hemorrhage in 12 midwest communities. J Stroke Cerebrovasc Dis. 2013; 23(5):1073-82. DOI: 10.1016/j.jstrokecerebrovasdis.2013.09.010. View

20.

Frieden C, Garai K . Concerning the structure of apoE. Protein Sci. 2013; 22(12):1820-5. PMC: 3843636. DOI: 10.1002/pro.2379. View