Reversible Self-Assembled Monolayers (rSAMs): Adaptable Surfaces for Enhanced Multivalent Interactions and Ultrasensitive Virus Detection

Overview

Journal ACS Cent Sci

Specialty Chemistry

Date 2017 Dec 5

PMID 29202022

Citations 7

Authors

Sing Yee Yeung

Annabell Mucha

Ravindra Deshmukh

Malak Boutrus

Thomas Arnebrant

Borje Sellergren

Affiliations

Soon will be listed here.

Abstract

We report on the design of pH-switchable monolayers allowing a reversible and ordered introduction of affinity reagents on sensor surfaces. The principal layer building blocks consist of α-(4-amidinophenoxy)alkanes decorated at the ω-position with affinity ligands. These spontaneously self-assemble on top of carboxylic acid terminated SAMs to form reversible homo or mixed monolayers (rSAMs) that are tunable with respect to the nature of the head group, layer order and stability while featuring pH responsiveness and the dynamic nature of noncovalent build assemblies. We show that this results in a range of unique biosensor features. As a first example a sialic acid rSAM featuring strong lectin affinity is here used to sense hemagglutinin and influenza virus (H5N1) at the pM and fM level by in situ ellipsometry in a fully reversible fashion. We believe that the rSAM concept will find widespread use in surface chemistry and overall for boosting sensitivity in affinity biosensors.

Citing Articles

Heteromultivalent Ligand Display on Reversible Self-Assembled Monolayers (rSAMs): A Fluidic Platform for Tunable Influenza Virus Recognition.

Sergeeva Y, Yeung S, Sellergren B ACS Appl Mater Interfaces. 2024; 16(3):3139-3146.

PMID: 38197122 PMC: 10811624. DOI: 10.1021/acsami.3c15699.

Aptamer-functionalized field-effect transistor biosensors for disease diagnosis and environmental monitoring.

Wang J, Chen D, Huang W, Yang N, Yuan Q, Yang Y Exploration (Beijing). 2023; 3(3):20210027.

PMID: 37933385 PMC: 10624392. DOI: 10.1002/EXP.20210027.

SAM-Support-Based Electrochemical Sensor for Aβ Biomarker Detection of Alzheimer's Disease.

Le P, Le H, Kim H, Cho S Biosensors (Basel). 2023; 13(8).

PMID: 37622895 PMC: 10452698. DOI: 10.3390/bios13080809.

SARS-CoV-2 spike proteins react with Au and Si, are electrically conductive and denature at 3 × 10 V m: a surface bonding and a single-protein circuit study.

Dief E, Darwish N Chem Sci. 2023; 14(13):3428-3440.

PMID: 37006686 PMC: 10055994. DOI: 10.1039/d2sc06492h.

Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior.

Yeung S, Sergeeva Y, Pan G, Mittler S, Ederth T, Dam T ACS Appl Mater Interfaces. 2022; 14(37):41790-41799.

PMID: 36074978 PMC: 9501787. DOI: 10.1021/acsami.2c12029.

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