Biosynthesis, Characterization, Antimicrobial and Cytotoxic Effects of Silver Nanoparticles Using Seed Extract

Overview

Journal Iran J Pharm Res

Publisher Brieflands

Specialty Pharmacology

Date 2017 Dec 5

PMID 29201104

Citations 24

Authors

Azam Chahardoli

Naser Karimi

Ali Fattahi

Affiliations

Soon will be listed here.

Abstract

The biogenic synthesis of metal nanomaterial offers an environmentally benign alternative to the traditional chemical synthesis routes. In the present study, the green synthesis of silver nanoparticles (AgNPs) from aqueous solution of silver nitrate (AgNO) by using L. seed powder extract (NSPE) has been reported. AgNPs were characterized by UV-vis absorption spectroscopy with an intense surface plasmon resonance band at 435 nm which reveals the formation of nanoparticles. Fourier transmission infrared spectroscopy (FTIR) showed that nanoparticles were capped with plant compounds. Transmission electron microscopy (TEM) showed silver nanoparticles, with a size of 2-15 nm, were spherical. The X-ray diffraction spectrum (XRD) pattern clearly indicates that AgNPs formed in the present synthesis were crystalline in nature. Stabilized films of exudate synthesized AgNPs were effective anti-bacterial agents. In addition, these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on a human breast cancer cell line (MCF-7) and a human colorectal adenocarcinoma cell line (HT-29). The results confirmed that the NSPE is a very good ecofriendly and nontoxic source for the synthesis of AgNPs as compared to the conventional chemical/physical methods. Therefore, seed provides future opportunities in nanomedicine by tagging nanoparticles with secondary metabolites.

Citing Articles

Impeding Biofilm-Forming Mediated Methicillin-Resistant and Virulence Genes Using a Biosynthesized Silver Nanoparticles-Antibiotic Combination.

Fareid M, El-Sherbiny G, Askar A, Abdelaziz A, Hegazy A, Ab Aziz R Biomolecules. 2025; 15(2).

PMID: 40001569 PMC: 11852608. DOI: 10.3390/biom15020266.

Impeding microbial biofilm formation and Pseudomonas aeruginosa virulence genes using biologically synthesized silver Carthamus nanoparticles.

Abdel-Fatah S, Mohammad N, Elshimy R, Mosallam F Microb Cell Fact. 2024; 23(1):240.

PMID: 39238019 PMC: 11378559. DOI: 10.1186/s12934-024-02508-9.

Therapeutic Potential of Aloe vera and Aloe vera-Conjugated Silver Nanoparticles on Mice Exposed to Hexavalent Chromium.

Nauroze T, Ali S, Andleeb S, Ara C, Liaqat I, Mushtaq H Biol Trace Elem Res. 2024; 202(12):5580-5595.

PMID: 38478315 DOI: 10.1007/s12011-024-04105-8.

Review green synthesis of silver nanoparticles by using plant extracts and their antimicrobial activity.

Abada E, Mashraqi A, Modafer Y, Al Abboud M, El-Shabasy A Saudi J Biol Sci. 2023; 31(1):103877.

PMID: 38148949 PMC: 10749906. DOI: 10.1016/j.sjbs.2023.103877.

Advances in Phytonanotechnology: A Plant-Mediated Green Synthesis of Metal Nanoparticles Using Plant Extracts and Their Antimicrobial and Anticancer Applications.

Thatyana M, Dube N, Kemboi D, Manicum A, Mokgalaka-Fleischmann N, Tembu J Nanomaterials (Basel). 2023; 13(19).

PMID: 37836257 PMC: 10574544. DOI: 10.3390/nano13192616.

References

Jung W, Koo H, Kim K, Shin S, Kim S, Park Y . Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Appl Environ Microbiol. 2008; 74(7):2171-8. PMC: 2292600. DOI: 10.1128/AEM.02001-07. View

Gopinath V, MubarakAli D, Priyadarshini S, Meera Priyadharsshini N, Thajuddin N, Velusamy P . Biosynthesis of silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids Surf B Biointerfaces. 2012; 96:69-74. DOI: 10.1016/j.colsurfb.2012.03.023. View

Kim J, Kuk E, Yu K, Kim J, Park S, Lee H . Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007; 3(1):95-101. DOI: 10.1016/j.nano.2006.12.001. View

Zamanai Taghizadeh Rabe S, Mahmoudi M, Ahi A, Emami S . Antiproliferative effects of extracts from Iranian Artemisia species on cancer cell lines. Pharm Biol. 2011; 49(9):962-9. DOI: 10.3109/13880209.2011.559251. View

Nel A, Xia T, Madler L, Li N . Toxic potential of materials at the nanolevel. Science. 2006; 311(5761):622-7. DOI: 10.1126/science.1114397. View

Asharani P, Mun G, Hande M, Valiyaveettil S . Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 2009; 3(2):279-90. DOI: 10.1021/nn800596w. View

Feng Q, Wu J, Chen G, Cui F, Kim T, Kim J . A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res. 2000; 52(4):662-8. DOI: 10.1002/1097-4636(20001215)52:4<662::aid-jbm10>3.0.co;2-3. View

Raja K, Saravanakumar A, Vijayakumar R . Efficient synthesis of silver nanoparticles from Prosopis juliflora leaf extract and its antimicrobial activity using sewage. Spectrochim Acta A Mol Biomol Spectrosc. 2012; 97:490-4. DOI: 10.1016/j.saa.2012.06.038. View

Ghareib M, Tahon M, Saif M, El-Sayed Abdallah W . Rapid Extracellular Biosynthesis of Silver Nanoparticles by Culture Supernatant. Iran J Pharm Res. 2017; 15(4):915-924. PMC: 5316272. View

10.

Foldbjerg R, Dang D, Autrup H . Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. Arch Toxicol. 2010; 85(7):743-50. DOI: 10.1007/s00204-010-0545-5. View

11.

Liu J, Sonshine D, Shervani S, Hurt R . Controlled release of biologically active silver from nanosilver surfaces. ACS Nano. 2010; 4(11):6903-13. PMC: 3004478. DOI: 10.1021/nn102272n. View

12.

Ahmad N, Sharma S, Alam M, Singh V, Shamsi S, Mehta B . Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B Biointerfaces. 2010; 81(1):81-6. DOI: 10.1016/j.colsurfb.2010.06.029. View

13.

Mittal A, Chisti Y, Banerjee U . Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv. 2013; 31(2):346-56. DOI: 10.1016/j.biotechadv.2013.01.003. View

14.

Sanpui P, Chattopadhyay A, Ghosh S . Induction of apoptosis in cancer cells at low silver nanoparticle concentrations using chitosan nanocarrier. ACS Appl Mater Interfaces. 2011; 3(2):218-28. DOI: 10.1021/am100840c. View

15.

Dhillon G, Brar S, Kaur S, Verma M . Green approach for nanoparticle biosynthesis by fungi: current trends and applications. Crit Rev Biotechnol. 2011; 32(1):49-73. DOI: 10.3109/07388551.2010.550568. View

16.

Lukman A, Gong B, Marjo C, Roessner U, Harris A . Facile synthesis, stabilization, and anti-bacterial performance of discrete Ag nanoparticles using Medicago sativa seed exudates. J Colloid Interface Sci. 2010; 353(2):433-44. DOI: 10.1016/j.jcis.2010.09.088. View

17.

Zhang W, Yao Y, Sullivan N, Chen Y . Modeling the primary size effects of citrate-coated silver nanoparticles on their ion release kinetics. Environ Sci Technol. 2011; 45(10):4422-8. DOI: 10.1021/es104205a. View

18.

Kim S, Choi J, Choi J, Chung K, Park K, Yi J . Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicol In Vitro. 2009; 23(6):1076-84. DOI: 10.1016/j.tiv.2009.06.001. View

19.

Prathna T, Chandrasekaran N, Raichur A, Mukherjee A . Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids Surf B Biointerfaces. 2010; 82(1):152-9. DOI: 10.1016/j.colsurfb.2010.08.036. View

20.

Yu S, Yin Y, Liu J . Silver nanoparticles in the environment. Environ Sci Process Impacts. 2014; 15(1):78-92. DOI: 10.1039/c2em30595j. View