Human Antibody-based Chemically Induced Dimerizers for Cell Therapeutic Applications

Overview

Journal Nat Chem Biol

Specialties Biochemistry
Biology
Chemistry

Date 2017 Dec 5

PMID 29200207

Citations 38

Authors

Zachary B Hill

Alexander J Martinko

Duy P Nguyen

James A Wells

Affiliations

Soon will be listed here.

Abstract

Chemically induced dimerizers (CIDs) have emerged as one of the most powerful tools for artificially regulating signaling pathways in cells; however, currently available CID systems lack the properties desired for use in regulating cellular therapies. Here, we report the development of human antibody-based chemically induced dimerizers (AbCIDs) from known small-molecule-protein complexes by selecting for synthetic antibodies that recognize the chemical epitope created by the bound small molecule. We demonstrate this concept by generating three antibodies that are highly selective for the BCL-xL-ABT-737 complex compared to BCL-xL alone. We show the potential of AbCIDs for application in regulating human cell therapies by using them to induce CRISPRa-mediated gene expression and to regulate CAR T-cell activation. We believe that the AbCIDs generated in this study will find application in regulating cell therapies and that the general method of AbCID development may lead to the creation of many new and orthogonal CIDs.

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