Restriction Endonuclease Cleavage Map and Location of Unique Features of the DNA of Hepatitis B Virus, Subtype Adw2

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Sep 1

PMID 291995

Citations 22

Authors

A Siddiqui

F Sattler

W S ROBINSON

Affiliations

Soon will be listed here.

Abstract

DNA of hepatitis B virus (HBV) of hepatitis B surface antigen (HBsAg) subtype adw2 made fully double stranded by the virion DNA polymerase and radiolabeled either by the virion DNA polymerase reaction or by nick-translation with 32P-labeled deoxynucleoside triphosphates was used to establish a map of restriction endonuclease cleavage sites by the method double and triple enzyme digestion and to determine the relative positions of several unique physical features of this DNA. The five restriction sites for enzyme HincII, the two sites each for BamHI, Ava I, and Bgl II, and the single sites for EcoRI, Pst I, Hpa I, and Taq I were positioned relative to each other. Within this map, the single-stranded region in HBV DNA has been localized and the locations of nicks in each strand (a and b) have been determined with respect to restriction sites on the circular map. Comparison of restriction endonuclease cleavage patterns of DNAs of HBV of HBsAg subtypes adw2, ayw3, and adrq+ revealed consistent differences among subtypes and occasional differences within subtypes.

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References

Almeida J, Rubenstein D, Stott E . New antigen-antibody system in Australia-antigen-positive hepatitis. Lancet. 1971; 2(7736):1225-7. DOI: 10.1016/s0140-6736(71)90543-5. View

ROBINSON W . The genome of hepatitis B virus. Annu Rev Microbiol. 1977; 31:357-77. DOI: 10.1146/annurev.mi.31.100177.002041. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Hruska J, Clayton D, Rubenstein J, ROBINSON W . Structure of hepatitis B Dane particle DNA before and after the Dane particle DNA polymerase reaction. J Virol. 1977; 21(2):666-72. PMC: 353869. DOI: 10.1128/JVI.21.2.666-672.1977. View

Landers T, Greenberg H, ROBINSON W . Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction. J Virol. 1977; 23(2):368-76. PMC: 515839. DOI: 10.1128/JVI.23.2.368-376.1977. View

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

Roberts R . Restriction endonucleases. CRC Crit Rev Biochem. 1976; 4(2):123-64. DOI: 10.3109/10409237609105456. View

Bancroft W, MUNDON F, RUSSELL P . Detection of additional antigenic determinants of hepatitis B antigen. J Immunol. 1972; 109(4):842-8. View

LE BOUVIER G . The heterogeneity of Australia antigen. J Infect Dis. 1971; 123(6):671-5. DOI: 10.1093/infdis/123.6.671. View

10.

SOULIER J . Further data on HBs antigen subtypes - geographical distribution. Vox Sang. 1974; 27(6):533-49. DOI: 10.1111/j.1423-0410.1974.tb02451.x. View

11.

DANE D, Cameron C, Briggs M . Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet. 1970; 1(7649):695-8. DOI: 10.1016/s0140-6736(70)90926-8. View

12.

Hedgpeth J, Goodman H, Boyer H . DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A. 1972; 69(11):3448-52. PMC: 389791. DOI: 10.1073/pnas.69.11.3448. View

13.

ROBINSON W, Clayton D, Greenman R . DNA of a human hepatitis B virus candidate. J Virol. 1974; 14(2):384-91. PMC: 355524. DOI: 10.1128/JVI.14.2.384-391.1974. View

14.

ROBINSON W, Greenman R . DNA polymerase in the core of the human hepatitis B virus candidate. J Virol. 1974; 13(6):1231-6. PMC: 355442. DOI: 10.1128/JVI.13.6.1231-1236.1974. View

15.

KAPLAN P, Greenman R, Gerin J, Purcell R, ROBINSON W . DNA polymerase associated with human hepatitis B antigen. J Virol. 1973; 12(5):995-1005. PMC: 356728. DOI: 10.1128/JVI.12.5.995-1005.1973. View

16.

Summers J, OConnell A, Millman I . Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles. Proc Natl Acad Sci U S A. 1975; 72(11):4597-601. PMC: 388770. DOI: 10.1073/pnas.72.11.4597. View

17.

Shih J, Gerin J . Immunochemistry of hepatitis B surface antigen (HBsAg): preparation and characterization of antibodies to the constituent polypeptides. J Immunol. 1975; 115(3):634-9. View