Microprinted Stem Cell Niches Reveal Compounding Effect of Colony Size on Stromal Cells-Mediated Neural Differentiation

Overview

Journal Adv Healthc Mater

Specialties Biomedical Engineering
Biotechnology

Date 2017 Dec 2

PMID 29193846

Citations 12

Authors

Ramila Joshi

Pradip Shahi Thakuri

James C Buchanan

Jun Li

Hossein Tavana

Affiliations

Soon will be listed here.

Abstract

Microenvironmental factors have a major impact on differentiation of embryonic stem cells (ESCs). Here, a novel phenomenon that size of ESC colonies has a significant regulatory role on stromal cells induced differentiation of ESCs to neural cells is reported. Using a robotic cell microprinting technology, defined densities of ESCs are confined within aqueous nanodrops over a layer of supporting stromal cells immersed in a second, immiscible aqueous phase to generate ESC colonies of defined sizes. Temporal protein and gene expression studies demonstrate that larger ESC colonies generate disproportionally more neural cells and longer neurite processes. Unlike previous studies that attribute neural differentiation of ESCs solely to interactions with stromal cells, it is found that increased intercellular signaling of ESCs significantly enhances neural differentiation. This study offers an approach to generate neural cells with improved efficiency for potential use in translational research.

Citing Articles

Connexin 25 maintains self-renewal and functions of airway basal cells for airway regeneration.

Zhang J, Wang S, Liu Z, Zhong C, Lei Y, Zheng Q Stem Cell Res Ther. 2024; 15(1):286.

PMID: 39256871 PMC: 11389295. DOI: 10.1186/s13287-024-03908-9.

Inhibiting BRAF/EGFR/MEK suppresses cancer stemness and drug resistance of primary colorectal cancer cells.

Lamichhane A, Luker G, Agarwal S, Tavana H Oncotarget. 2023; 14:879-889.

PMID: 37791907 PMC: 10549774. DOI: 10.18632/oncotarget.28517.

Therapeutic Targeting of Cancer Stem Cells Prevents Resistance of Colorectal Cancer Cells to MEK Inhibition.

Lamichhane A, Shahi Thakuri P, Singh S, Rafsanjani Nejad P, Heiss J, Luker G ACS Pharmacol Transl Sci. 2022; 5(9):724-734.

PMID: 36110381 PMC: 9469186. DOI: 10.1021/acsptsci.1c00257.

Therapeutic Targeting of Stromal-Tumor HGF-MET Signaling in an Organotypic Triple-Negative Breast Tumor Model.

Singh S, Lamichhane A, Rafsanjani Nejad P, Heiss J, Baumann H, Gudneppanavar R Mol Cancer Res. 2022; 20(7):1166-1177.

PMID: 35348758 PMC: 9262791. DOI: 10.1158/1541-7786.MCR-21-0317.

Cell-Inspired All-Aqueous Microfluidics: From Intracellular Liquid-Liquid Phase Separation toward Advanced Biomaterials.

Ma Q, Song Y, Sun W, Cao J, Yuan H, Wang X Adv Sci (Weinh). 2020; 7(7):1903359.

PMID: 32274317 PMC: 7141073. DOI: 10.1002/advs.201903359.

References

Tropepe V, Sibilia M, Ciruna B, Rossant J, Wagner E, van der Kooy D . Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev Biol. 1999; 208(1):166-88. DOI: 10.1006/dbio.1998.9192. View

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

Joshi R, Buchanan J, Tavana H . Self-regulatory factors of embryonic stem cells in co-culture with stromal cells enhance neural differentiation. Integr Biol (Camb). 2017; 9(5):418-426. PMC: 5498101. DOI: 10.1039/c7ib00038c. View

Cui X, Dean D, Ruggeri Z, Boland T . Cell damage evaluation of thermal inkjet printed Chinese hamster ovary cells. Biotechnol Bioeng. 2010; 106(6):963-9. DOI: 10.1002/bit.22762. View

Matthys O, Hookway T, McDevitt T . Design Principles for Engineering of Tissues from Human Pluripotent Stem Cells. Curr Stem Cell Rep. 2016; 2(1):43-51. PMC: 4910633. DOI: 10.1007/s40778-016-0030-z. View

Parnas D, Linial M . Acceleration of neuronal maturation of P19 cells by increasing culture density. Brain Res Dev Brain Res. 1997; 101(1-2):115-24. DOI: 10.1016/s0165-3806(97)00057-6. View

Lindvall O, Kokaia Z, Martinez-Serrano A . Stem cell therapy for human neurodegenerative disorders-how to make it work. Nat Med. 2004; 10 Suppl:S42-50. DOI: 10.1038/nm1064. View

Lutolf M, Gilbert P, Blau H . Designing materials to direct stem-cell fate. Nature. 2009; 462(7272):433-41. PMC: 2908011. DOI: 10.1038/nature08602. View

Banda E, Grabel L . Directed Differentiation of Human Embryonic Stem Cells into Neural Progenitors. Methods Mol Biol. 2014; 1307:289-98. DOI: 10.1007/7651_2014_67. View

10.

Bauwens C, Peerani R, Niebruegge S, Woodhouse K, Kumacheva E, Husain M . Control of human embryonic stem cell colony and aggregate size heterogeneity influences differentiation trajectories. Stem Cells. 2008; 26(9):2300-10. DOI: 10.1634/stemcells.2008-0183. View

11.

Papalopulu N, Clarke J, Bradley L, Wilkinson D, Krumlauf R, Holder N . Retinoic acid causes abnormal development and segmental patterning of the anterior hindbrain in Xenopus embryos. Development. 1991; 113(4):1145-58. DOI: 10.1242/dev.113.4.1145. View

12.

Juliandi B, Abematsu M, Nakashima K . Epigenetic regulation in neural stem cell differentiation. Dev Growth Differ. 2010; 52(6):493-504. DOI: 10.1111/j.1440-169X.2010.01175.x. View

13.

Fu J, Wang Y, Yang M, Desai R, Yu X, Liu Z . Mechanical regulation of cell function with geometrically modulated elastomeric substrates. Nat Methods. 2010; 7(9):733-6. PMC: 3069358. DOI: 10.1038/nmeth.1487. View

14.

Discher D, Mooney D, Zandstra P . Growth factors, matrices, and forces combine and control stem cells. Science. 2009; 324(5935):1673-7. PMC: 2847855. DOI: 10.1126/science.1171643. View

15.

Schwanhausser B, Busse D, Li N, Dittmar G, Schuchhardt J, Wolf J . Global quantification of mammalian gene expression control. Nature. 2011; 473(7347):337-42. DOI: 10.1038/nature10098. View

16.

Abranches E, Silva M, Pradier L, Schulz H, Hummel O, Henrique D . Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo. PLoS One. 2009; 4(7):e6286. PMC: 2709448. DOI: 10.1371/journal.pone.0006286. View

17.

Ross H, Ambrosio F, Trumbower R, Reier P, Behrman A, Wolf S . Neural Stem Cell Therapy and Rehabilitation in the Central Nervous System: Emerging Partnerships. Phys Ther. 2016; 96(5):734-42. PMC: 6281018. DOI: 10.2522/ptj.20150063. View

18.

Wade A, McKinney A, Phillips J . Matrix regulators in neural stem cell functions. Biochim Biophys Acta. 2014; 1840(8):2520-5. PMC: 4074566. DOI: 10.1016/j.bbagen.2014.01.017. View

19.

Cohen D, Malone E, Lipson H, Bonassar L . Direct freeform fabrication of seeded hydrogels in arbitrary geometries. Tissue Eng. 2006; 12(5):1325-35. DOI: 10.1089/ten.2006.12.1325. View

20.

Perrimon N, Pitsouli C, Shilo B . Signaling mechanisms controlling cell fate and embryonic patterning. Cold Spring Harb Perspect Biol. 2012; 4(8):a005975. PMC: 3405863. DOI: 10.1101/cshperspect.a005975. View