Transcriptomics Reveal an Integrative Role for Maternal Thyroid Hormones During Zebrafish Embryogenesis

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 2

PMID 29192226

Citations 7

Authors

Nadia Silva

Bruno Louro

Marlene Trindade

Deborah M Power

Marco A Campinho

Affiliations

Soon will be listed here.

Abstract

Thyroid hormones (THs) are essential for embryonic brain development but the genetic mechanisms involved in the action of maternal THs (MTHs) are still largely unknown. As the basis for understanding the underlying genetic mechanisms of MTHs regulation we used an established zebrafish monocarboxylic acid transporter 8 (MCT8) knock-down model and characterised the transcriptome in 25hpf zebrafish embryos. Subsequent mapping of differentially expressed genes using Reactome pathway analysis together with in situ expression analysis and immunohistochemistry revealed the genetic networks and cells under MTHs regulation during zebrafish embryogenesis. We found 4,343 differentially expressed genes and the Reactome pathway analysis revealed that TH is involved in 1681 of these pathways. MTHs regulated the expression of core developmental pathways, such as NOTCH and WNT in a cell specific context. The cellular distribution of neural MTH-target genes demonstrated their cell specific action on neural stem cells and differentiated neuron classes. Taken together our data show that MTHs have a role in zebrafish neurogenesis and suggest they may be involved in cross talk between key pathways in neural development. Given that the observed MCT8 zebrafish knockdown phenotype resembles the symptoms in human patients with Allan-Herndon-Dudley syndrome our data open a window into understanding the genetics of this human congenital condition.

Citing Articles

In a zebrafish biomedical model of human Allan-Herndon-Dudley syndrome impaired MTH signaling leads to decreased neural cell diversity.

Silva N, Campinho M Front Endocrinol (Lausanne). 2023; 14:1157685.

PMID: 37214246 PMC: 10194031. DOI: 10.3389/fendo.2023.1157685.

The Role of Estrogen and Thyroid Hormones in Zebrafish Visual System Function.

Cohen A, Popowitz J, Delbridge-Perry M, Rowe C, Connaughton V Front Pharmacol. 2022; 13:837687.

PMID: 35295340 PMC: 8918846. DOI: 10.3389/fphar.2022.837687.

Genetic and Neurological Deficiencies in the Visual System of Mutant Zebrafish.

Rozenblat R, Tovin A, Zada D, Lebenthal-Loinger I, Lerer-Goldshtein T, Appelbaum L Int J Mol Sci. 2022; 23(5).

PMID: 35269606 PMC: 8910067. DOI: 10.3390/ijms23052464.

Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models.

Song J, Lu Y, Cheng X, Shi C, Lou Q, Jin X Cells. 2021; 10(8).

PMID: 34440752 PMC: 8391828. DOI: 10.3390/cells10081984.

Changes in thyroid hormone activity disrupt photomotor behavior of larval zebrafish.

Walter K, Miller G, Chen X, Harvey D, Puschner B, Lein P Neurotoxicology. 2019; 74:47-57.

PMID: 31121238 PMC: 6750994. DOI: 10.1016/j.neuro.2019.05.008.

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