Translation and Characterization of Messenger RNAs in Differentiating Chicken Cartilage

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1979 Oct 1

PMID 291903

Citations 23

Authors

W B Upholt

B M Vertel

A DORFMAN

Affiliations

Soon will be listed here.

Abstract

Total RNA, prepared from chicken limb bud cultures undergoing differentiation to cartilage, has been translated in a wheat germ cell-free protein-synthesizing system. Antibodies against chondroitin sulfate proteoglycan core protein immunoprecipitate a single component which migrates as a protein of 340,000 daltons in sodium dodecyl sulfate/polyacrylamide gels. The messenger RNA for this protein sediments at approximately 27 S in 70% formamide or aqueous sucrose gradients. The 340,000-dalton protein is present in cell-free translation products directed by RNA prepared from limb bud cultures and sternae and is absent in cell-free translation directed by RNA prepared from embryonic calvaria or liver. The level of synthesis of this protein is greatly reduced when RNA prepared from limb bud cultures inhibited from differentiation by BrdUrd is used. (Pre)pro alpha 1(I), -alpha 2(I), and -alpha 1(II) collagen bands have been identified on gels by electrophoresis of collagenase-digested or immunoprecipitated cell-free translation products directed by RNA from differentiating limb bud cultures, embryonic sternae, and embryonic calvaria.

Citing Articles

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The chondrodystrophy, nanomelia: biosynthesis and processing of the defective aggrecan precursor.

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