Molecular Characterization of Recovered from Traditional Milk Products in Kashan, Iran

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2017 Nov 30

PMID 29184374

Citations 9

Authors

Farhad Sharafati Chaleshtori

Navid Mazroii Arani

Esmat Aghadavod

Afshin Naseri

Reza Sharafati Chaleshtori

Affiliations

Soon will be listed here.

Abstract

Aim: Shiga toxigenic (STEC) strains as emerging groups of foodborne pathogens are responsible for most foodborne illnesses. The aim of this study was to determine the antibiotic resistance pattern in STEC isolated from traditional milk products and their molecular characterization.

Materials And Methods: A total of 116 samples were randomly purchased from local markets in Kashan, Iran, and evaluated for the occurrence of STEC by culturing and molecular methods. The antibiotic resistance of obtained isolates was determined by Kirby Bauer method. Furthermore, isolates were assayed for the presence of Shiga toxins ( and ) and intimin gene ().

Results: The incidence of in 60 ice cream, 30 yoghurt, and 26 cheese samples was 8.33%, 10%, and 11.54%, respectively. The findings showed that 11 out of 11 (100%) had both and while gene was not found in isolated of traditional milk products. For strains carrying and , highest antibiotic sensitive levels were related to trimethoprim/sulfamethoxazole, norfloxacin, chloramphenicol, and ciprofloxacin, respectively.

Conclusion: The results showed relationship between the presence of virulence factors and antimicrobial resistance. These results can be used for further studies on STEC as an emerging foodborne pathogen.

Citing Articles

Occurrence of diarrheagenic Escherichia coli pathotypes from raw milk and unpasteurized buttermilk by culture and multiplex polymerase chain reaction in southwest Iran.

Ahmady A, Aliyan Aliabadi R, Amin M, Ameri A, Abbasi Montazeri E Mol Biol Rep. 2023; 50(4):3661-3667.

PMID: 36808583 DOI: 10.1007/s11033-023-08261-3.

Occurrence, Pathogenic Potential and Antimicrobial Resistance of Isolated from Raw Milk Cheese Commercialized in Banat Region, Romania.

Imre K, Ban-Cucerzan A, Herman V, Sallam K, Cristina R, Abd-Elghany S Antibiotics (Basel). 2022; 11(6).

PMID: 35740128 PMC: 9220297. DOI: 10.3390/antibiotics11060721.

Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of Isolated from Milk and Dairy Products in Isfahan, Iran.

Madani A, Esfandiari Z, Shoaei P, Ataei B Foods. 2022; 11(7).

PMID: 35407047 PMC: 8997477. DOI: 10.3390/foods11070960.

Occurrence and D-Tryptophan Application for Controlling the Growth of Multidrug-Resistant Non-O157 Shiga Toxin-Producing in Dairy Products.

Elafify M, Sadoma N, Abd El Aal S, Bayoumi M, Ismail T Animals (Basel). 2022; 12(7).

PMID: 35405910 PMC: 8996961. DOI: 10.3390/ani12070922.

Loads of Coliforms and Fecal Coliforms and Characterization of Thermotolerant in Fresh Raw Milk Cheese.

Hammad A, Eltahan A, Hassan H, Abbas N, Hussien H, Shimamoto T Foods. 2022; 11(3).

PMID: 35159482 PMC: 8834472. DOI: 10.3390/foods11030332.

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