A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2017 Nov 29

PMID 29182568

Citations 3

Authors

Ornella Maglio

Salvatore Costanzo

Rosaria Cercola

Gerardo Zambrano

Marco Mauro

Raffaele Battaglia

Gianluca Ferrini

Flavia Nastri

Vincenzo Pavone

Angela Lombardi

Affiliations

Soon will be listed here.

Abstract

A cost-effective immunosensor for the detection and isolation of dental pulp stem cells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specific monoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCM surface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin-avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with a mixed cell population, was analyzed; the results indicated that the developed QCM immunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell from samples with high complexity, being simple, selective, and providing for more convenient and time-saving operations.

Citing Articles

Practical Use of Quartz Crystal Microbalance Monitoring in Cartilage Tissue Engineering.

Naranda J, Bracic M, Vogrin M, Maver U, Trojner T J Funct Biomater. 2022; 13(4).

PMID: 36278628 PMC: 9590066. DOI: 10.3390/jfb13040159.

Removal of Thiol-SAM on a Gold Surface for Re-Use of an Interdigitated Chain-Shaped Electrode.

Le H, Phan L, Cho S Materials (Basel). 2022; 15(6).

PMID: 35329670 PMC: 8950519. DOI: 10.3390/ma15062218.

Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials.

Zambrano G, Ruggiero E, Malafronte A, Chino M, Maglio O, Pavone V Int J Mol Sci. 2018; 19(10).

PMID: 30250002 PMC: 6213134. DOI: 10.3390/ijms19102896.

References

Wink T, van Zuilen S, Bult A, van Bennkom W . Self-assembled monolayers for biosensors. Analyst. 1997; 122(4):43R-50R. DOI: 10.1039/a606964i. View

Masdor N, Altintas Z, Tothill I . Sensitive detection of Campylobacter jejuni using nanoparticles enhanced QCM sensor. Biosens Bioelectron. 2015; 78:328-336. DOI: 10.1016/j.bios.2015.11.033. View

Speight R, Cooper M . A survey of the 2010 quartz crystal microbalance literature. J Mol Recognit. 2012; 25(9):451-73. DOI: 10.1002/jmr.2209. View

Chang J, Zhang C, Tani-Ishii N, Shi S, Wang C . NF-kappaB activation in human dental pulp stem cells by TNF and LPS. J Dent Res. 2005; 84(11):994-8. PMC: 1764452. DOI: 10.1177/154405910508401105. View

Toma J, Akhavan M, Fernandes K, Sadikot A, Kaplan D, Miller F . Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol. 2001; 3(9):778-84. DOI: 10.1038/ncb0901-778. View

Shen H, Zhou T, Hu J . A high-throughput QCM chip configuration for the study of living cells and cell-drug interactions. Anal Bioanal Chem. 2017; 409(27):6463-6473. DOI: 10.1007/s00216-017-0591-4. View

Ashri N, Ajlan S, Aldahmash A . Dental pulp stem cells. Biology and use for periodontal tissue engineering. Saudi Med J. 2015; 36(12):1391-9. PMC: 4707394. DOI: 10.15537/smj.2015.12.12750. View

Diamandis E, Christopoulos T . The biotin-(strept)avidin system: principles and applications in biotechnology. Clin Chem. 1991; 37(5):625-36. View

Potdar P, Jethmalani Y . Human dental pulp stem cells: Applications in future regenerative medicine. World J Stem Cells. 2015; 7(5):839-51. PMC: 4478630. DOI: 10.4252/wjsc.v7.i5.839. View

10.

Fathi F, Rahbarghazi R, Rashidi M . Label-free biosensors in the field of stem cell biology. Biosens Bioelectron. 2017; 101:188-198. DOI: 10.1016/j.bios.2017.10.028. View

11.

Yakovleva M, Safina G, Danielsson B . A study of glycoprotein-lectin interactions using quartz crystal microbalance. Anal Chim Acta. 2010; 668(1):80-5. DOI: 10.1016/j.aca.2009.12.004. View

12.

Yam C, Pradier C, Salmain M, Marcus P, Jaouen G . Binding of Biotin to Gold Surfaces Functionalized by Self-Assembled Monolayers of Cystamine and Cysteamine: Combined FT-IRRAS and XPS Characterization. J Colloid Interface Sci. 2001; 235(1):183-189. DOI: 10.1006/jcis.2000.7362. View

13.

Su X, Li Y . A self-assembled monolayer-based piezoelectric immunosensor for rapid detection of Escherichia coli O157:H7. Biosens Bioelectron. 2003; 19(6):563-74. DOI: 10.1016/s0956-5663(03)00254-9. View

14.

Battye F, Shortman K . Flow cytometry and cell-separation procedures. Curr Opin Immunol. 1991; 3(2):238-41. DOI: 10.1016/0952-7915(91)90058-9. View

15.

Cagnin S, Cimetta E, Guiducci C, Martini P, Lanfranchi G . Overview of micro- and nano-technology tools for stem cell applications: micropatterned and microelectronic devices. Sensors (Basel). 2012; 12(11):15947-82. PMC: 3522993. DOI: 10.3390/s121115947. View

16.

Azzaroni O, Mir M, Knoll W . Supramolecular architectures of streptavidin on biotinylated self-assembled monolayers. Tracking biomolecular reorganization after bioconjugation. J Phys Chem B. 2007; 111(48):13499-503. DOI: 10.1021/jp076707q. View

17.

Della Ventura B, Sakac N, Funari R, Velotta R . Flexible immunosensor for the detection of salivary α-amylase in body fluids. Talanta. 2017; 174:52-58. DOI: 10.1016/j.talanta.2017.05.075. View

18.

Henningson Jr C, Stanislaus M, Gewirtz A . 28. Embryonic and adult stem cell therapy. J Allergy Clin Immunol. 2003; 111(2 Suppl):S745-53. DOI: 10.1067/mai.2003.133. View

19.

Della Ventura B, Iannaccone M, Funari R, Pica Ciamarra M, Altucci C, Capparelli R . Effective antibodies immobilization and functionalized nanoparticles in a quartz-crystal microbalance-based immunosensor for the detection of parathion. PLoS One. 2017; 12(2):e0171754. PMC: 5300251. DOI: 10.1371/journal.pone.0171754. View

20.

Seifert M, Rinke M, Galla H . Characterization of streptavidin binding to biotinylated, binary self-assembled thiol monolayers--influence of component ratio and solvent. Langmuir. 2010; 26(9):6386-93. DOI: 10.1021/la904087s. View