The O-specific Polysaccharide Lyase from the Phage LKA1 Tailspike Reduces Pseudomonas Virulence

Overview

Journal Sci Rep

Specialty Science

Date 2017 Nov 28

PMID 29176754

Citations 60

Authors

Tomasz Olszak

Mikhail M Shneider

Agnieszka Latka

Barbara Maciejewska

Christopher Browning

Lada V Sycheva

Anneleen Cornelissen

Katarzyna Danis-Wlodarczyk

Sofya N Senchenkova

Alexander S Shashkov

Grzegorz Gula

Michal Arabski

Slawomir Wasik

Konstantin A Miroshnikov

Rob Lavigne

Petr G Leiman

Yuriy A Knirel

Zuzanna Drulis-Kawa

Affiliations

Soon will be listed here.

Abstract

Pseudomonas phage LKA1 of the subfamily Autographivirinae encodes a tailspike protein (LKA1gp49) which binds and cleaves B-band LPS (O-specific antigen, OSA) of Pseudomonas aeruginosa PAO1. The crystal structure of LKA1gp49 catalytic domain consists of a beta-helix, an insertion domain and a C-terminal discoidin-like domain. The putative substrate binding and processing site is located on the face of the beta-helix whereas the C-terminal domain is likely involved in carbohydrates binding. NMR spectroscopy and mass spectrometry analyses of degraded LPS (OSA) fragments show an O5 serotype-specific polysaccharide lyase specificity. LKA1gp49 reduces virulence in an in vivo Galleria mellonella infection model and sensitizes P. aeruginosa to serum complement activity. This enzyme causes biofilm degradation and does not affect the activity of ciprofloxacin and gentamicin. This is the first comprehensive report on LPS-degrading lyase derived from a Pseudomonas phage. Biological properties reveal a potential towards its applications in antimicrobial design and as a microbiological or biotechnological tool.

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