Differentiation of Human Adipose-derived Stem Cells into Cardiomyocyte-like Cells in Fibrin Scaffold by a Histone Deacetylase Inhibitor

Overview

Journal Biomed Eng Online

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2017 Nov 25

PMID 29169361

Citations 7

Authors

Zahra Bagheri-Hosseinabadi

Parvin Salehinejad

Seyed Alireza Mesbah-Namin

Affiliations

Soon will be listed here.

Abstract

Background: Human adipose-derived stem cells (hADSCs) are capable of differentiating into many cells such as cardiac cells. Different types of inducers are used for cardiac cell differentiation, but this question still remains to be investigated, which one is the best. The aim of this paper was to investigate the effect of combination of fibrin scaffold and trichostatin A (TSA), for differentiation of hADSCs into cardiomyocyte-like cells.

Methods: After approval of characteristics of hADSCs and fibrin scaffold, hADSCs were cultured in fibrin scaffold with 10 µM TSA for 72 h and kept in standard conditions for 4 weeks. QRT-PCR and immunostaining assay were performed for evaluating the expression pattern of special cardiac genes and proteins.

Results: In particular, our study showed that fibrin scaffold alongside TSA enhanced expression of the selected genes and proteins.

Conclusions: We concluded that the TSA alone or with fibrin scaffold can lead to the generation of cardiac like cells in a short period of time.

Citing Articles

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Cardiac patches made of brown adipose-derived stem cell sheets and conductive electrospun nanofibers restore infarcted heart for ischemic myocardial infarction.

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Mishan M, Balagholi S, Chamani T, Feizi S, Soheili Z, Kanavi M Cell Tissue Bank. 2021; 23(1):171-183.

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