Lack of Teratogenic and Mutagenic Effects of Nitrite on Mouse Fetuses

To assess embryotoxic effects of sodium nitrite, pregnant ICR mice were given drinking water containing sodium nitrite at a concentration of either 100 or 1000 mg/L on days 7-18 of gestation. There were no significant differences between treated and control groups in measures of developmental toxicity, e.g., litter size, fetal weight, and number of resorbed or dead fetuses. The incidences of external and skeletal malformations in fetuses of treated groups were not significantly different from those in the controls. No significant increase was observed in the frequency of gaps and breaks of liver cell chromosomes in fetuses exposed in utero to sodium nitrite. Teratogenic and mutagenic effects of sodium nitrite were absent in mice at the doses used.