[Study on Genetype in Lung Squamous Carcinoma by High-throughput of  Transcriptome Sequence]

Overview

Journal Zhongguo Fei Ai Za Zhi

Specialties Oncology
Pulmonary Medicine

Date 2017 Nov 24

PMID 29167000

Citations 3

Authors

Ying Xiong

Mingzhen Li

Panpan Zhang

Liyi Zhang

Yue Yang

Affiliations

Soon will be listed here.

Abstract

Background: Recently, there is no effective targeted drug for to the lung squamous carcinoma, the targeted drug for the adenocarcinoma does not benefit for the patients with squamous lung cancer, and researchers have known less targets for this type of patients. The main study herein is to screen and identify the specific key genes for the lung squamous carcinoma that will provide potential novel targets to describe pathogenesis of lung cancer.

Methods: Transcript sequence was used to detect the five pairs lung squamous carcinoma and normal lung tissues and bioinformatics was performed to investigate the differential coding genes between them and quantitative PCR was done to validate the key genes expression in lung cancer cell lines.

Results: The results of transcriptome sequence showed that 534 genes were up-regulated in the tumor tissues compared with the corresponding normal tissues. The top ten increased genes in the tumors were GAGE12J, SPRR3, PRAME, SPRR1A, SPRR2E, MAGEA3, SPRR1B, IL36G and TMPRSS11D. Next, we identified expression of SPRR family in lung cancer cell lines H520, GLC82, A549, H1299 and PC9. And we found higher expression of SPRR family genes in H1299 cells which metastasized to the lymph node.

Conclusions: SPRR family genes are identified to be highly expressed in the lung squamous carcinoma by high throughput transcript sequencing. This SPRR family genes are associated with lymph node metastasis that provide novel idea for lung cancer therapy. .

Citing Articles

Small Proline-Rich Protein 2B Facilitates Gastric Adenocarcinoma Proliferation via MDM2-p53/p21 Signaling Pathway.

Yao L, Yan J, Cheng F, Gan L, Huang Y, Zheng L Onco Targets Ther. 2021; 14:1453-1463.

PMID: 33664578 PMC: 7924129. DOI: 10.2147/OTT.S281032.

Identification of small proline-rich protein 1B (SPRR1B) as a prognostically predictive biomarker for lung adenocarcinoma by integrative bioinformatic analysis.

Zhang Z, Shi R, Xu S, Li Y, Zhang H, Liu M Thorac Cancer. 2021; 12(6):796-806.

PMID: 33501784 PMC: 7952803. DOI: 10.1111/1759-7714.13836.

The Role of Cavin3 in the Progression of Lung Cancer and Its Mechanism.

Sun G, Ni K Biomed Res Int. 2020; 2020:6364801.

PMID: 32352004 PMC: 7178469. DOI: 10.1155/2020/6364801.

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[Study on Genetype in Lung Squamous Carcinoma by High-throughput of Transcriptome Sequence]

[Study on Genetype in Lung Squamous Carcinoma by High-throughput of  Transcriptome Sequence]