Design and Testing of an Experimental Steam-Induced Burn Model in Rats

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2017 Nov 22

PMID 29159185

Citations 6

Authors

Vlad Porumb

Alexandru Florentin Trandabat

Cristina Terinte

Irina Draga Caruntu

Elena Porumb-Andrese

Mihail Gabriel Dimofte

Dragos Pieptu

Affiliations

Soon will be listed here.

Abstract

Background: Most of the current models for experimental burns pose difficulties in ensuring consistency and standardization.

Aim Of Study: We aimed to develop an automated, reproducible technique for experimental burns using steam-based heat transfer.

Methods: The system developed for steam exposure was based on a novel, integrated, computer-controlled design. Three groups of rats were exposed to steam for 1, 3, and 7 seconds. The lesions were evaluated after 20 minutes, 48 hours, and 72 hours after burn induction.

Results: One-second steam application produced a superficial second-degree burn; three-second application induced deep second-degree burn; and seven-second application led to a third-degree burn.

Conclusion: The high level of automation of our integrated, computer-controlled system makes the difference between our system and other models, by ensuring the control of the duration of exposure, temperature, and pressure and eliminating as many potential human generated errors as possible. The automated system can accurately reproduce specific types of burns, according to histological assessment. This model could generate the reproducible data needed in the study of burn pathology and in order to assess new treatments.

Citing Articles

Current understanding of thermo(dys)regulation in severe burn injury and the pathophysiological influence of hypermetabolism, adrenergic stress and hypothalamic regulation-a systematic review.

Mertin V, Most P, Busch M, Trojan S, Tapking C, Haug V Burns Trauma. 2022; 10:tkac031.

PMID: 36168403 PMC: 9501704. DOI: 10.1093/burnst/tkac031.

Healing Effects of Human Amniotic Membrane and Burned Wool on the Second-degree Burn in Rats.

Tanideh N, Keshavarzi F, Hemat Zadeh A, Daneshi S, Koohi-Hosseinabadi O, Mokhtari M Galen Med J. 2021; 9:e1759.

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Effects of Fibroblast Growth Factor 2 on Burn Injury and Repair Process: Analysis Using a Refined Mouse Model.

Hishida K, Hatano S, Furukawa H, Yokoo K, Watanabe H Plast Reconstr Surg Glob Open. 2020; 8(4):e2757.

PMID: 32440425 PMC: 7209900. DOI: 10.1097/GOX.0000000000002757.

Evaluation of wound healing potential of pomegranate () whole fruit extract on skin burn wound in rats ().

Lukiswanto B, Miranti A, Sudjarwo S, Primarizky H, Yuniarti W J Adv Vet Anim Res. 2019; 6(2):202-207.

PMID: 31453192 PMC: 6702873. DOI: 10.5455/javar.2019.f333.

Effects and parameters of the photobiomodulation in experimental models of third-degree burn: systematic review.

Ocon C, Dos Santos S, Caires J, de Oliveira M, Serra A, Leal-Junior E Lasers Med Sci. 2018; 34(3):637-648.

PMID: 30229346 DOI: 10.1007/s10103-018-2633-3.

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