Adenosine Deaminase Activity in Pig Saliva: Analytical Validation of Two Spectrophotometric Assays

Overview

Journal J Vet Diagn Invest

Specialty Veterinary Medicine

Date 2017 Nov 18

PMID 29145785

Citations 20

Authors

Fernando Tecles

Camila P Rubio

Maria D Contreras-Aguilar

Marina Lopez-Arjona

Silvia Martinez-Miro

Silvia Martinez-Subiela

Jose J Ceron

Affiliations

Soon will be listed here.

Abstract

We validated 2 assays for the measurement of adenosine deaminase (ADA) activity in the saliva of pigs: the Giusti-Galanti manual method (ADA-GG) and a commercial automated assay (Diazyme Laboratories; ADA-D). Intra-assay coefficients of variation (CVs) were <7 and 9%, and interassay CVs were <12 and 5%, for ADA-GG and ADA-D, respectively. Accuracy was measured by 2 methods: recovery and linearity-under-dilution. Recovery was 82.4-106.8% for ADA-GG, and 92.8-107.9% for ADA-D. Serial dilutions showed R > 0.95 and 0.99 for ADA-GG and ADA-D, respectively. Linear regression between the methods gave R = 0.997 ( p < 0.0001), and a Bland-Altman plot showed a proportional bias of 112 IU/L (95% confidence interval of -99 to 322 IU/L) for ADA-D. No significant differences were observed between the results obtained by either method in saliva or serum. ADA activity was much higher in porcine saliva than in serum. Salivary ADA activity was significantly higher in lame pigs compared to healthy animals. However, serum ADA activity was significantly lower in lame pigs.

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References

Adams A, HARKNESS R . Adenosine deaminase activity in thymus and other human tissues. Clin Exp Immunol. 1976; 26(3):647-9. PMC: 1540957. View

Kose K, Yazici C, Assioglu O . The evaluation of lipid peroxidation and adenosine deaminase activity in patients with Behçet's disease. Clin Biochem. 2001; 34(2):125-9. DOI: 10.1016/s0009-9120(01)00190-4. View

Calis M, Ates F, Yazici C, Kose K, Kirnap M, Demir M . Adenosine deaminase enzyme levels, their relation with disease activity, and the effect of colchicine on adenosine deaminase levels in patients with Behçet's disease. Rheumatol Int. 2005; 25(6):452-6. DOI: 10.1007/s00296-005-0612-z. View

Saracoglu U, Guven O, Durak I . Adenosine deaminase and 5'-nucleotidase activities in saliva from patients with oral and laryngeal cancer. Oral Dis. 2005; 11(5):323-5. DOI: 10.1111/j.1601-0825.2005.01131.x. View

Baganha M, Pego A, Lima M, Gaspar E, Cordeiro A . Serum and pleural adenosine deaminase. Correlation with lymphocytic populations. Chest. 1990; 97(3):605-10. DOI: 10.1378/chest.97.3.605. View

Bansal S, Singh R, Narang R, Joshi L, Bansal A, Agrawal A . Serum adenosine deaminase in pulmonary tuberculosis, malignancy and non-tubercular respiratory diseases. Indian J Chest Dis Allied Sci. 1991; 33(4):189-93. View

Rai B, Kaur J, Jacobs R, Anand S . Adenosine deaminase in saliva as a diagnostic marker of squamous cell carcinoma of tongue. Clin Oral Investig. 2010; 15(3):347-9. DOI: 10.1007/s00784-010-0404-z. View

Beyazit Y, Koklu S, Tas A, Purnak T, Sayilir A, Kurt M . Serum adenosine deaminase activity as a predictor of disease severity in ulcerative colitis. J Crohns Colitis. 2012; 6(1):102-7. DOI: 10.1016/j.crohns.2011.07.010. View

Gutierrez A, Nobauer K, Soler L, Razzazi-Fazeli E, Gemeiner M, Ceron J . Detection of potential markers for systemic disease in saliva of pigs by proteomics: a pilot study. Vet Immunol Immunopathol. 2012; 151(1-2):73-82. DOI: 10.1016/j.vetimm.2012.10.007. View

10.

Andreasson U, Perret-Liaudet A, van Waalwijk van Doorn L, Blennow K, Chiasserini D, Engelborghs S . A Practical Guide to Immunoassay Method Validation. Front Neurol. 2015; 6:179. PMC: 4541289. DOI: 10.3389/fneur.2015.00179. View

11.

Tonin A, Calado A, Bottari N, Dalenogare D, Thome G, Duarte T . Novel markers of inflammatory response and hepatic dysfunction in canine leishmaniasis. Comp Immunol Microbiol Infect Dis. 2015; 44:61-4. DOI: 10.1016/j.cimid.2015.09.004. View

12.

Vijayamahantesh , Amit A, Dikhit M, Pandey R, Singh K, Mishra R . Elevated Serum ADA Activity as a Marker for Diagnosis and Prognosis of Visceral Leishmaniasis and Post Kala-Azar Dermal Leishmaniasis in Indian Patients. PLoS One. 2016; 11(5):e0154117. PMC: 4871472. DOI: 10.1371/journal.pone.0154117. View

13.

Sullivan J, Osborne W, WEDGEWOOD R . Adenosine deaminase activity in lymphocytes. Br J Haematol. 1977; 37(1):157-8. View

14.

Macdermott R, TRITSCH G, Formeister J . Adenosine deaminase and nucleoside phosphorylase activities in normal human blood mononuclear cell subpopulations. Clin Exp Immunol. 1980; 42(2):303-7. PMC: 1537102. View

15.

Iizuka H, Koizumi H, Kamigaki K, Aoyagi T, Miura Y . Two forms of adenosine deaminase in pig epidermis. J Dermatol. 1981; 8(2):91-5. DOI: 10.1111/j.1346-8138.1981.tb02573.x. View

16.

Galanti B, Nardiello S, Russo M, Fiorentino F . Increased lymphocyte adenosine deaminase in typhoid fever. Scand J Infect Dis. 1981; 13(1):47-50. DOI: 10.1080/00365548.1981.11690366. View

17.

Piras M, Gakis C, Budroni M, Andreoni G . Adenosine deaminase activity in pleural effusions: an aid to differential diagnosis. Br Med J. 1978; 2(6154):1751-2. PMC: 1610017. DOI: 10.1136/bmj.2.6154.1751-a. View

18.

Mishra O, Gupta B, Ali Z, Nath G, Chandra L . Adenosine deaminase activity in typhoid fever. Indian Pediatr. 1994; 31(11):1379-84. View