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Maintained MPF Level After Oocyte Vitrification Improves Embryonic Development After IVF, but Not After Somatic Cell Nuclear Transfer

Overview
Journal Mol Cells
Publisher Elsevier
Date 2017 Nov 18
PMID 29145719
Citations 4
Authors
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Abstract

Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. Here, we evaluated MPF activity in vitrified mouse eggs following treatment with caffeine, a known stimulator of MPF activity, and/or the proteasome inhibitor MG132. Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG). After warming, the MPF activity of oocytes and their blastocyst formation and implantation rates in the CA, MG, and CA+MG groups were much higher than those in the untreated group. However, the cell numbers in blastocysts did not differ among groups. Analysis of the effectiveness of caffeine and MG132 for improving somatic cell nuclear transfer (SCNT) technology using cryopreserved eggs showed that supplementation did not improve the blastocyst formation rate of cloned mouse eggs. These results suggest that maintaining MPF activity after cryopreservation may have a positive effect on further embryonic development, but is unable to fully overcome cryoinjury. Thus, intrinsic factors governing the developmental potential that diminish during oocyte cryopreservation should be explored.

Citing Articles

The effect of vitrification after warming on the expressions of p38, CDK1, and cyclin B in immature goat oocytes followed by maturation.

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PMID: 33281346 PMC: 7704326. DOI: 10.14202/vetworld.2020.2126-2132.


Elevation of MPF and MAPK gene expression, GSH content and mitochondrial distribution quality induced by melatonin promotes porcine oocyte maturation and development in vitro.

Zhao Z, Yang L, Zhang D, Zheng Z, Li N, Li Q PeerJ. 2020; 8:e9913.

PMID: 33083108 PMC: 7543723. DOI: 10.7717/peerj.9913.


Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes.

Lee A, Hong K, Choi S, Park C, Park J, Il Lee J Stem Cell Reports. 2019; 12(3):545-556.

PMID: 30799275 PMC: 6411484. DOI: 10.1016/j.stemcr.2019.01.019.


Caffeine and oocyte vitrification: Sheep as an animal model.

Moawad A, Choi I, Zhu J, El-Wishy A, Amarnath D, Chen W Int J Vet Sci Med. 2019; 6(Suppl):S41-S48.

PMID: 30761320 PMC: 6161861. DOI: 10.1016/j.ijvsm.2018.01.004.

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