Protection by Nitrite Against the Ischemic Effects Induced by Acute Myocardial Infarction in Mice

Overview

Journal Anatol J Cardiol

Publisher Kare Publishing

Specialty Cardiology & Vascular Diseases

Date 2017 Nov 18

PMID 29145236

Citations 3

Authors

Yafei Wang

Xin Song

Xia Yue

Hong Su

Yuanliang Gu

Linda Bowman

Min Ding

Baobo Zou

Jinshun Zhao

Xialu Lin

Affiliations

Soon will be listed here.

Abstract

Objective: This research was aimed to investigate the correct dose of nitrite that would act as a protection against the ischemic effects induced by acute myocardial infarction (AMI).

Methods: Mice were randomly divided into a sham-operation group (sham), an AMI operation group (AMI), and a nitrite pretreatment+AMI operation group (N+AMI). Seven days before the AMI operation, mice in the N+AMI group were pretreated with sodium nitrite in drinking water.

Results: One week after the AMI operation, serum lactate dehydrogenase (LDH) and creatine kinase (CK) activities in both AMI and N+AMI group were significantly higher than those in the sham group, but there were no significant differences between AMI and N+AMI mice. Contents of inducible nitric oxide synthase (iNOS) in the noninfarct area of the left ventricle in the N+AMI mice were significantly higher than those in the AMI mice, with no difference in the infarct area. Coagulation necrosis in the cardiomyocytes was observed in both AMI and N+AMI mice; however, it was less severe in the N+AMI mice. Western blot analyses showed that nitrite pretreatment resulted in up-regulation of antiapoptotic factors Bcl-2 and p21waf1/cip1 signal proteins, but down-regulation of the proapoptotic factor Bax signal protein. Furthermore, nitrite pretreatment also showed significant alleviation of AMI-induced signal protein expressions of inflammatory factors of NF-K B and oxidative factors of Hsp 70 and HO-1.

Conclusion: These results suggest that nitrite show certain protective effects against the ischemic effects induced by AMI in mice, which might be attributed to the synthesis of NO induced by iNOS through up-regulation of antiapoptotic factors and down-regulation of proapoptotic and inflammatory factors.

Citing Articles

Soluble guanylate cyclase (sGC) stimulator vericiguat alleviates myocardial ischemia-reperfusion injury by improving microcirculation.

Cai Y, Zhang B, Shalamu A, Gao T, Ge J Ann Transl Med. 2022; 10(12):662.

PMID: 35845490 PMC: 9279818. DOI: 10.21037/atm-22-2583.

Effect of pantoprazole on I-R-induced myocardial injury in diabetic rats targeting inflammatory cytokine release and oxidative stress.

Taneja G, Sharma A, Khanna D, Rajput S Iran J Basic Med Sci. 2021; 24(5):615-622.

PMID: 34249262 PMC: 8244611. DOI: 10.22038/ijbms.2021.51624.11714.

Different Changing Patterns of Three NOS-NO System Activities after Ischemia-Reperfusion in Rabbit with AMI.

Zhang J, Chen C, Li F, Gao G, Fu J, Niu H Cell Biochem Biophys. 2021; 79(4):857-862.

PMID: 34125429 DOI: 10.1007/s12013-021-01011-x.

References

Lundberg J, Weitzberg E, Gladwin M . The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nat Rev Drug Discov. 2008; 7(2):156-67. DOI: 10.1038/nrd2466. View

Becker T, Mevius I, de Vries D, Schaapherder A, Zu Vilsendorf A, Klempnauer J . The L-arginine/NO pathway in end-stage liver disease and during orthotopic liver and kidney transplantation: biological and analytical ramifications. Nitric Oxide. 2008; 20(1):61-7. DOI: 10.1016/j.niox.2008.10.002. View

Webb A, Bond R, McLean P, Uppal R, Benjamin N, Ahluwalia A . Reduction of nitrite to nitric oxide during ischemia protects against myocardial ischemia-reperfusion damage. Proc Natl Acad Sci U S A. 2004; 101(37):13683-8. PMC: 518813. DOI: 10.1073/pnas.0402927101. View

Bhushan S, Kondo K, Polhemus D, Otsuka H, Nicholson C, Tao Y . Nitrite therapy improves left ventricular function during heart failure via restoration of nitric oxide-mediated cytoprotective signaling. Circ Res. 2014; 114(8):1281-91. PMC: 4943032. DOI: 10.1161/CIRCRESAHA.114.301475. View

Iwanaga N, Yamamasu S, Tachibana D, Nishio J, Nakai Y, Shintaku H . Activity of synthetic enzymes of tetrahydrobiopterin in the human placenta. Int J Mol Med. 2003; 13(1):117-20. View

Sindler A, Cox-York K, Reese L, Bryan N, Seals D, Gentile C . Oral nitrite therapy improves vascular function in diabetic mice. Diab Vasc Dis Res. 2015; 12(3):221-4. PMC: 5027127. DOI: 10.1177/1479164114565628. View

Duranski M, Greer J, Dejam A, Jaganmohan S, Hogg N, Langston W . Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver. J Clin Invest. 2005; 115(5):1232-40. PMC: 1077170. DOI: 10.1172/JCI22493. View

Bryan N, Calvert J, Elrod J, Gundewar S, Ji S, Lefer D . Dietary nitrite supplementation protects against myocardial ischemia-reperfusion injury. Proc Natl Acad Sci U S A. 2007; 104(48):19144-9. PMC: 2141922. DOI: 10.1073/pnas.0706579104. View

Cho S, Delehedde M, RODRIGUEZ-VILLANUEVA J, Brisbay S, McDonnell T . Bax gene disruption alters the epidermal response to ultraviolet irradiation and in vivo induced skin carcinogenesis. Int J Mol Med. 2001; 7(3):235-41. DOI: 10.3892/ijmm.7.3.235. View

10.

Hendgen-Cotta U, Merx M, Shiva S, Schmitz J, Becher S, Klare J . Nitrite reductase activity of myoglobin regulates respiration and cellular viability in myocardial ischemia-reperfusion injury. Proc Natl Acad Sci U S A. 2008; 105(29):10256-61. PMC: 2481313. DOI: 10.1073/pnas.0801336105. View

11.

Chiarugi V, Magnelli L, Gallo O . Cox-2, iNOS and p53 as play-makers of tumor angiogenesis (review). Int J Mol Med. 1998; 2(6):715-9. DOI: 10.3892/ijmm.2.6.715. View

12.

Hou Y, Janczuk A, Wang P . Current trends in the development of nitric oxide donors. Curr Pharm Des. 1999; 5(6):417-41. View

13.

Abueid L, Uslu U, Cumbul A, Ogunc A, Ercan F, Alican I . Inhibition of 5-lipoxygenase by zileuton in a rat model of myocardial infarction. Anatol J Cardiol. 2016; 17(4):269-275. PMC: 5469106. DOI: 10.14744/AnatolJCardiol.2016.7248. View

14.

Aktan F . iNOS-mediated nitric oxide production and its regulation. Life Sci. 2004; 75(6):639-53. DOI: 10.1016/j.lfs.2003.10.042. View

15.

Sindler A, Fleenor B, Calvert J, Marshall K, Zigler M, Lefer D . Nitrite supplementation reverses vascular endothelial dysfunction and large elastic artery stiffness with aging. Aging Cell. 2011; 10(3):429-37. PMC: 3094511. DOI: 10.1111/j.1474-9726.2011.00679.x. View

16.

Muller D, Dechend R, Mervaala E, Park J, Schmidt F, Fiebeler A . NF-kappaB inhibition ameliorates angiotensin II-induced inflammatory damage in rats. Hypertension. 2000; 35(1 Pt 2):193-201. DOI: 10.1161/01.hyp.35.1.193. View

17.

Zuckerbraun B, Shiva S, Ifedigbo E, Mathier M, Mollen K, Rao J . Nitrite potently inhibits hypoxic and inflammatory pulmonary arterial hypertension and smooth muscle proliferation via xanthine oxidoreductase-dependent nitric oxide generation. Circulation. 2009; 121(1):98-109. DOI: 10.1161/CIRCULATIONAHA.109.891077. View

18.

Israsena N, Kessler J . Msx2 and p21(CIP1/WAF1) mediate the proapoptotic effects of bone morphogenetic protein-4 on ventricular zone progenitor cells. J Neurosci Res. 2002; 69(6):803-9. DOI: 10.1002/jnr.10362. View

19.

Shiva S, Sack M, Greer J, Duranski M, Ringwood L, Burwell L . Nitrite augments tolerance to ischemia/reperfusion injury via the modulation of mitochondrial electron transfer. J Exp Med. 2007; 204(9):2089-102. PMC: 2118713. DOI: 10.1084/jem.20070198. View

20.

Basanez G, Soane L, Hardwick J . A new view of the lethal apoptotic pore. PLoS Biol. 2012; 10(9):e1001399. PMC: 3457931. DOI: 10.1371/journal.pbio.1001399. View