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Analytical Study of Effective Biodegradation of -cresol Using ABHI001: Application in Bioremediation

Overview
Journal 3 Biotech
Publisher Springer
Specialty Biotechnology
Date 2017 Nov 17
PMID 29142804
Citations 2
Authors
Tripti Singh
Neha Srivastava
A K Bhatiya
P K Mishra
Affiliations
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Abstract

This study evaluated the capability of ABHI001 to effectively degrade -cresol through different techniques. The molecular identity of the laboratory isolate ABHI001 was confirmed through the 16S ribosomal DNA gene pattern, and its morphological features were investigated through field-emission scanning electron microscopy. In addition, the degradation behavior of the isolate for cresol was verified using several techniques, including UV-visible spectroscopy, followed by high-performance liquid chromatography (HPLC), gas chromatography, and Fourier transform infrared spectroscopy. The maximum degradation percentage of 85% for -cresol could be achieved after 18 h of incubation with . ABHI001. The formation of -hydroxybenzaldehyde, -hydroxybenzoate, and protocatechuate metabolites was confirmed through HPLC. The study results indicate that . ABHI001 may have applications in the bioremediation of organic pollutants, such as -cresol.

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