MiR-93-3p Inhibition Suppresses Clear Cell Renal Cell Carcinoma Proliferation, Metastasis and Invasion

Overview

Journal Oncotarget

Specialty Oncology

Date 2017 Nov 16

PMID 29137305

Citations 12

Authors

Lu Wang

Guang Yang

Xiangwei Zhu

Ziqi Wang

Hongzhi Wang

Yang Bai

Pengcheng Sun

Li Peng

Wei Wei

Guang Chen

Guangbin Li

Andrey A Zamyatnin Jr

Peter V Glybochko

Wanhai Xu

Affiliations

Soon will be listed here.

Abstract

miRNA dysregulation is associated with many human diseases, including cancer. This study explored the effects of miR-93-3p on clear cell renal cell carcinoma (ccRCC). We found that miR-93-3p is upregulated an average of 38-fold in 138 ccRCC specimens compared to matched normal kidney tissues, which correlated with poor patient outcome. miR-93-3p inhibition reduced ccRCC cell growth, invasion, and migration and in a mouse xenograft model. A search of the TargetScan, miRanda, and PicTar databases revealed that miR-93-3p is predicted to regulate pigment epithelium-derived factor (PEDF). A direct PEDF-miR-93-3p interaction was confirmed via dual-luciferase reporter assays. Like miR-93-3p inhibition, PEDF overexpression induced cell apoptosis and inhibited migration and invasion. Additionally, co-transfection with PEDF siRNA reversed the effects of miR-93-3p inhibition in ccRCC cells. Thus, miR-93-3p is a likely ccRCC oncogene that acts by regulating PEDF. These results suggest that miR-93-3p may predict ccRCC patient clinical outcome and serve as a novel anti-ccRCC therapeutic target.

Citing Articles

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