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Detection of Antigens in Urine of Mice and Humans Infected with Borrelia Burgdorferi, Etiologic Agent of Lyme Disease

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1989 Jan 1
PMID 2913036
Citations 26
Authors
F W Hyde
R C Johnson
T J White
C E Shelburne
Affiliations
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Abstract

Lyme disease is a seasonal tick-borne malady which has worldwide distribution. Early and accurate diagnosis of Lyme disease is essential for successful antibiotic therapy. Symptoms are too vague to make an early diagnosis based on conventional criteria. We report the detection of antigens of Borrelia burgdorferi, causative agent of Lyme disease, in the urine of infected mice and humans. This technique may eventually provide a rapid diagnostic test for the early and accurate detection of this illness.

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References
1.
Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View
2.
Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View
3.
Galfre G, Milstein C . Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981; 73(Pt B):3-46. DOI: 10.1016/0076-6879(81)73054-4. View
4.
Steere A, Grodzicki R, Kornblatt A, Craft J, Barbour A, BURGDORFER W . The spirochetal etiology of Lyme disease. N Engl J Med. 1983; 308(13):733-40. DOI: 10.1056/NEJM198303313081301. View
5.
Russell H, Sampson J, Schmid G, WILKINSON H, Plikaytis B . Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis. 1984; 149(3):465-70. DOI: 10.1093/infdis/149.3.465. View