Optimization of Physical Conditions for the Production of Thermostable T1 Lipase in Pichia Guilliermondii Strain SO Using Response Surface Methodology

Overview

Journal BMC Biotechnol

Publisher Biomed Central

Specialty Biotechnology

Date 2017 Nov 12

PMID 29126403

Citations 8

Authors

Mary Ladidi Abu

Hisham Mohd Nooh

Siti Nurbaya Oslan

Abu Bakar Salleh

Affiliations

Soon will be listed here.

Abstract

Background: Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the screening and optimisation of physical conditions for T1 lipase production in P. guilliermondii.

Result: The screening of six physical conditions by Plackett-Burman Design has identified pH, inoculum size and incubation time as exerting significant effects on lipase production. These three conditions were further optimised using, Box-Behnken Design of Response Surface Methodology, which predicted an optimum medium comprising pH 6, 24 h incubation time and 2% inoculum size. T1 lipase activity of 2.0 U/mL was produced with a biomass of OD 23.0.

Conclusion: The process of using RSM for optimisation yielded a 3-fold increase of T1 lipase over medium before optimisation. Therefore, this result has proven that T1 lipase can be produced at a higher yield in P. guilliermondii.

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