Construction of P-glycoprotein Incorporated Tethered Lipid Bilayer Membranes

Overview

Journal Biochem Biophys Rep

Specialty Biochemistry

Date 2017 Nov 11

PMID 29124152

Citations 10

Authors

Fatih Inci

Umit Celik

Basak Turken

Hakan Ozgur Ozer

Fatma Nese Kok

Affiliations

Soon will be listed here.

Abstract

To investigate drug-membrane protein interactions, an artificial tethered lipid bilayer system was constructed for the functional integration of membrane proteins with large extra-membrane domains such as multi-drug resistance protein 1 (MDR1). In this study, a modified lipid (, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol)-2000] (DSPE-PEG)) was utilized as a spacer molecule to elevate lipid membrane from the sensor surface and generate a reservoir underneath. Concentration of DSPE-PEG molecule significantly affected the liposome binding/spreading and lipid bilayer formation, and 0.03 mg/mL of DSPE-PEG provided optimum conditions for membrane protein integration. Further, the incorporation of MDR1 increased the local rigidity on the platform. Antibody binding studies showed the functional integration of MDR1 protein into lipid bilayer platform. The platform allowed to follow MDR!-statin-based drug interactions . Each binding event and lipid bilayer formation was monitored in real-time using Surface Plasmon Resonance and Quartz Crystal Microbalance-Dissipation systems, and Atomic Force Microscopy was used for visualization experiments.

Citing Articles

In situ synthesis and dynamic simulation of molecularly imprinted polymeric nanoparticles on a micro-reactor system.

Erdem O, Es I, Saylan Y, Atabay M, Gungen M, Olmez K Nat Commun. 2023; 14(1):4840.

PMID: 37563147 PMC: 10415298. DOI: 10.1038/s41467-023-40413-8.

Aptamer-Based Point-of-Care Devices: Emerging Technologies and Integration of Computational Methods.

Aslan Y, Atabay M, Chowdhury H, Gokturk I, Saylan Y, Inci F Biosensors (Basel). 2023; 13(5).

PMID: 37232930 PMC: 10216294. DOI: 10.3390/bios13050569.

The complex relationship between multiple drug resistance and the tumor pH gradient: a review.

Koltai T Cancer Drug Resist. 2022; 5(2):277-303.

PMID: 35800371 PMC: 9255250. DOI: 10.20517/cdr.2021.134.

Smart materials-integrated sensor technologies for COVID-19 diagnosis.

Erdem O, Derin E, Sagdic K, Yilmaz E, Inci F Emergent Mater. 2021; 4(1):169-185.

PMID: 33495747 PMC: 7817967. DOI: 10.1007/s42247-020-00150-w.

Advances in Biomimetic Systems for Molecular Recognition and Biosensing.

Saylan Y, Erdem O, Inci F, Denizli A Biomimetics (Basel). 2020; 5(2).

PMID: 32408710 PMC: 7345028. DOI: 10.3390/biomimetics5020020.

References

Reimhult E, Zach M, Hook F, Kasemo B . A multitechnique study of liposome adsorption on Au and lipid bilayer formation on SiO2. Langmuir. 2006; 22(7):3313-9. DOI: 10.1021/la0519554. View

Atanasov V, Knorr N, Duran R, Ingebrandt S, Offenhausser A, Knoll W . Membrane on a chip: a functional tethered lipid bilayer membrane on silicon oxide surfaces. Biophys J. 2005; 89(3):1780-8. PMC: 1366681. DOI: 10.1529/biophysj.105.061374. View

Milhiet P, Gubellini F, Berquand A, Dosset P, Rigaud J, le Grimellec C . High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer. Biophys J. 2006; 91(9):3268-75. PMC: 1614478. DOI: 10.1529/biophysj.106.087791. View

Allen C, Dos Santos N, Gallagher R, Chiu G, Shu Y, Li W . Controlling the physical behavior and biological performance of liposome formulations through use of surface grafted poly(ethylene glycol). Biosci Rep. 2002; 22(2):225-50. DOI: 10.1023/a:1020186505848. View

Peetla C, Stine A, Labhasetwar V . Biophysical interactions with model lipid membranes: applications in drug discovery and drug delivery. Mol Pharm. 2009; 6(5):1264-76. PMC: 2757518. DOI: 10.1021/mp9000662. View

Cho N, Frank C, Kasemo B, Hook F . Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates. Nat Protoc. 2010; 5(6):1096-106. DOI: 10.1038/nprot.2010.65. View

Hopfner M, Rothe U, Bendas G . Biosensor-based evaluation of liposomal behavior in the target binding process. J Liposome Res. 2008; 18(1):71-82. DOI: 10.1080/08982100801894000. View

Munro J, Frank C . In situ formation and characterization of poly(ethylene glycol)-supported lipid bilayers on gold surfaces. Langmuir. 2004; 20(24):10567-75. DOI: 10.1021/la048378o. View

Lissandrello C, Inci F, Francom M, Paul M, Demirci U, Ekinci K . Nanomechanical motion of adhered to a surface. Appl Phys Lett. 2014; 105(11):113701. PMC: 4187256. DOI: 10.1063/1.4895132. View

10.

Keller C, Kasemo B . Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance. Biophys J. 1998; 75(3):1397-1402. PMC: 1299813. DOI: 10.1016/S0006-3495(98)74057-3. View

11.

Shafiee H, Lidstone E, Jahangir M, Inci F, Hanhauser E, Henrich T . Nanostructured optical photonic crystal biosensor for HIV viral load measurement. Sci Rep. 2014; 4:4116. PMC: 3937800. DOI: 10.1038/srep04116. View

12.

Bayburt T, Sligar S . Membrane protein assembly into Nanodiscs. FEBS Lett. 2009; 584(9):1721-7. PMC: 4758813. DOI: 10.1016/j.febslet.2009.10.024. View

13.

Anger G, Cressman A, Piquette-Miller M . Expression of ABC Efflux transporters in placenta from women with insulin-managed diabetes. PLoS One. 2012; 7(4):e35027. PMC: 3338746. DOI: 10.1371/journal.pone.0035027. View

14.

Wang S, Esfahani M, Gurkan U, Inci F, Kuritzkes D, Demirci U . Efficient on-chip isolation of HIV subtypes. Lab Chip. 2012; 12(8):1508-15. PMC: 3777392. DOI: 10.1039/c2lc20706k. View

15.

Ieiri I . Functional significance of genetic polymorphisms in P-glycoprotein (MDR1, ABCB1) and breast cancer resistance protein (BCRP, ABCG2). Drug Metab Pharmacokinet. 2011; 27(1):85-105. DOI: 10.2133/dmpk.dmpk-11-rv-098. View

16.

Cho N, Kanazawa K, Glenn J, Frank C . Employing two different quartz crystal microbalance models to study changes in viscoelastic behavior upon transformation of lipid vesicles to a bilayer on a gold surface. Anal Chem. 2007; 79(18):7027-35. DOI: 10.1021/ac0709504. View

17.

Evans K . Supported phospholipid bilayer interaction with components found in typical room-temperature ionic liquids - a QCM-D and AFM Study. Int J Mol Sci. 2009; 9(4):498-511. PMC: 2635696. DOI: 10.3390/ijms9040498. View

18.

Jokerst J, Lobovkina T, Zare R, Gambhir S . Nanoparticle PEGylation for imaging and therapy. Nanomedicine (Lond). 2011; 6(4):715-28. PMC: 3217316. DOI: 10.2217/nnm.11.19. View

19.

Arnell R, Ferraz N, Fornstedt T . Analytical characterization of chiral drug-protein interactions: comparison between the optical biosensor (surface plasmon resonance) assay and the HPLC perturbation method. Anal Chem. 2006; 78(5):1682-9. DOI: 10.1021/ac051802l. View

20.

Sinner E, Reuning U, Kok F, Sacca B, Moroder L, Knoll W . Incorporation of integrins into artificial planar lipid membranes: characterization by plasmon-enhanced fluorescence spectroscopy. Anal Biochem. 2004; 333(2):216-24. DOI: 10.1016/j.ab.2004.05.022. View