Comparison of Femtosecond Laser-assisted Corneal Intrastromal Xenotransplantation and the Allotransplantation in Rhesus Monkeys

Overview

Journal BMC Ophthalmol

Publisher Biomed Central

Specialty Ophthalmology

Date 2017 Nov 11

PMID 29121878

Citations 9

Authors

He Jin

Liangping Liu

Hui Ding

Miao He

Chi Zhang

Xingwu Zhong

Affiliations

Soon will be listed here.

Abstract

Background: In our previous study, we showed that both allogeneic and autogeneic small-incision femtosecond laser-assisted corneal intrastromal transplantation are safe and effective surgeries. However, the results of small-incision femtosecond laser-assisted intrastromal xenotransplantation have not yet been explored. Additionally, we suggest that glycerol-dehydrated corneal lamellae might provide a possible alternative for this xenogenic implantation approach.

Methods: Corneal inlay lamellae were produced from rabbits and humans using femtosecond laser-assisted surgeries and were dehydrated in glycerol for 1 week at 4 °C. These xenogeneic glycerol-dehydrated grafts and fresh allogeneic monkey lamellae were then implanted into rhesus monkeys using small-incision femtosecond laser assistance. Postoperatively, clinical examinations, AS-OCT measurements and tear inflammatory mediator assays were performed.

Results: There were no significant changes in the transparency of the corneal lamellae after glycerol dehydration. Following implantation, no evidence of tissue rejection or severe inflammatory responses was observed in the monkeys, and the host corneas remained transparent throughout a 6-month observation period. The grafts were clearly visible via AS-OCT. Corneal thickness increased 1 week postoperatively but subsequently declined and remained unchanged 1 month after surgery. Significant changes were observed in all tear inflammatory mediators in the 'Rabbit to Monkey' group. The trends in changes of tear inflammatory mediators in the 'Human to Monkey' group were similar to those in the 'Rabbit to Monkey' group. At 1 month post-surgery, the levels of most tear inflammatory mediators had decreased, with the exception of IL-1β, TGF-β1 and IFN-γ in the allotransplantation group.

Conclusion: Small-incision femtosecond laser-assisted intrastromal transplantation minimized invasiveness and improved surgical efficiency. In addition, the host cornea maintained a high level of biocompatibility. Glycerol-dehydrated corneal lamellae might be potentially useful as an alternative inlay xenogeneic material. In this study, we also describe a new treatment that can be used in keratoconus, corneal ectasia, presbyopia, hyperpresbyopia and other diseases.

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References

Ganesh S, Brar S, Patel U . Comparison of ReLEx SMILE and PRK in terms of visual and refractive outcomes for the correction of low myopia. Int Ophthalmol. 2017; 38(3):1147-1154. DOI: 10.1007/s10792-017-0575-6. View

Feng Y, Wang W . In vivo confocal microscopic observation of lamellar corneal transplantation in the rabbit using xenogenic acellular corneal scaffolds as a substitute. Chin Med J (Engl). 2015; 128(7):933-40. PMC: 4834011. DOI: 10.4103/0366-6999.154301. View

Bian F, Barbosa F, Corrales R, Pelegrino F, Volpe E, Pflugfelder S . Altered balance of interleukin-13/interferon-gamma contributes to lacrimal gland destruction and secretory dysfunction in CD25 knockout model of Sjögren's syndrome. Arthritis Res Ther. 2015; 17:53. PMC: 4392623. DOI: 10.1186/s13075-015-0582-9. View

Holzer M, Rabsilber T, Auffarth G . Femtosecond laser-assisted corneal flap cuts: morphology, accuracy, and histopathology. Invest Ophthalmol Vis Sci. 2006; 47(7):2828-31. DOI: 10.1167/iovs.05-1123. View

Blum M, Taubig K, Gruhn C, Sekundo W, Kunert K . Five-year results of Small Incision Lenticule Extraction (ReLEx SMILE). Br J Ophthalmol. 2016; 100(9):1192-5. DOI: 10.1136/bjophthalmol-2015-306822. View

Haber M, Cao Z, Panjwani N, Bedenice D, Li W, Provost P . Effects of growth factors (EGF, PDGF-BB and TGF-beta 1) on cultured equine epithelial cells and keratocytes: implications for wound healing. Vet Ophthalmol. 2003; 6(3):211-7. DOI: 10.1046/j.1463-5224.2003.00296.x. View

KING Jr J, McTIGUE J, MERYMAN H . A simple method of preservation of corneas for lamellar keratoplasty. Am J Ophthalmol. 1962; 53:445-9. DOI: 10.1016/0002-9394(62)94873-0. View

Zhang Y, Wu S, Yao Y . Long-term comparison of full-bed deep anterior lamellar keratoplasty and penetrating keratoplasty in treating keratoconus. J Zhejiang Univ Sci B. 2013; 14(5):438-50. PMC: 3650457. DOI: 10.1631/jzus.B1200272. View

Yoo S, Hurmeric V . Femtosecond laser-assisted keratoplasty. Am J Ophthalmol. 2011; 151(2):189-91. DOI: 10.1016/j.ajo.2010.10.008. View

10.

Spadea L, De Rosa V . Current techniques of lamellar keratoplasty for keratoconus. Saudi Med J. 2016; 37(2):127-36. PMC: 4800909. DOI: 10.15537/smj.2016.2.12985. View

11.

Afzali B, Lombardi G, Lechler R, Lord G . The role of T helper 17 (Th17) and regulatory T cells (Treg) in human organ transplantation and autoimmune disease. Clin Exp Immunol. 2007; 148(1):32-46. PMC: 1868863. DOI: 10.1111/j.1365-2249.2007.03356.x. View

12.

Wedner S, Dineen B . Refractive errors. Trop Doct. 2003; 33(4):207-9. DOI: 10.1177/004947550303300406. View

13.

Chen W, Lin Y, Zhang X, Wang L, Liu M, Liu J . Comparison of fresh corneal tissue versus glycerin-cryopreserved corneal tissue in deep anterior lamellar keratoplasty. Invest Ophthalmol Vis Sci. 2009; 51(2):775-81. DOI: 10.1167/iovs.09-3422. View

14.

Utine C, Tzu J, Akpek E . Lamellar keratoplasty using gamma-irradiated corneal lenticules. Am J Ophthalmol. 2010; 151(1):170-174.e1. DOI: 10.1016/j.ajo.2010.08.007. View

15.

Li J, Yu L, Deng Z, Wang L, Sun L, Ma H . Deep anterior lamellar keratoplasty using acellular corneal tissue for prevention of allograft rejection in high-risk corneas. Am J Ophthalmol. 2011; 152(5):762-70.e3. DOI: 10.1016/j.ajo.2011.05.002. View

16.

de Paiva C, Chotikavanich S, Pangelinan S, Pitcher 3rd J, Fang B, Zheng X . IL-17 disrupts corneal barrier following desiccating stress. Mucosal Immunol. 2009; 2(3):243-53. PMC: 3594767. DOI: 10.1038/mi.2009.5. View

17.

Li J, Shi S, Zhang X, Ni S, Wang Y, Curcio C . Comparison of different methods of glycerol preservation for deep anterior lamellar keratoplasty eligible corneas. Invest Ophthalmol Vis Sci. 2012; 53(9):5675-85. DOI: 10.1167/iovs.12-9936. View

18.

Dana M, Qian Y, Hamrah P . Twenty-five-year panorama of corneal immunology: emerging concepts in the immunopathogenesis of microbial keratitis, peripheral ulcerative keratitis, and corneal transplant rejection. Cornea. 2000; 19(5):625-43. DOI: 10.1097/00003226-200009000-00008. View

19.

Fontana L, Errani P, Zerbinati A, Musacchi Y, Di Pede B, Tassinari G . Frequency of positive donor rim cultures after penetrating keratoplasty using hypothermic and organ-cultured donor corneas. Cornea. 2007; 26(5):552-6. DOI: 10.1097/ICO.0b013e3180415d7e. View

20.

Tripathi H, Mehdi M, Gupta D, Sen S, Kashyap S, Nag T . Long-term preservation of donor corneas in glycerol for keratoplasty: exploring new protocols. Br J Ophthalmol. 2015; 100(2):284-90. DOI: 10.1136/bjophthalmol-2015-306944. View