Microscopic Differentiation of Plasmonic Nanoparticles for the Ratiometric Read-out of Target DNA

Overview

Journal Sci Rep

Specialty Science

Date 2017 Nov 9

PMID 29116199

Citations 2

Authors

Zhenjie Wu

Rui Yang

Di Zu

Shuqing Sun

Affiliations

Soon will be listed here.

Abstract

The development of highly sensitive and rapid methods for detecting DNA is of critical importance. Here, we describe a strategy for the digital detection of target DNA at the femto-molar level. Individual DNA molecules were encoded with a single gold nanorod (AuNR), separated and enriched by magnetic immune-separation. The coding gold nanorods were then de-hybridized and dispersed into a gold nanosphere (AuNS) solution at a certain concentration, and both gold nanoparticles were immobilized on glass slides for dark-field microscopic imaging. Using an in-house Matlab program, the concentration of the target DNA was calculated based on the ratio of the coding gold nanorods to gold nanospheres. By combining the coding of individual biomolecules with a single gold nanorod and the use of gold nanospheres as an internal standard, a method for the rapid and accurate digital detection of target DNA to the femto-molar level was developed.

Citing Articles

An aptasensor based on the microscopic enumeration of encoding gold nanoparticles for the detection of C-reactive protein.

Zhao Y, Zhao J, Jin T, Sun S, Liu W, Tan Y RSC Adv. 2022; 9(59):34293-34298.

PMID: 35529987 PMC: 9073860. DOI: 10.1039/c9ra06203c.

Dark Field Microscopy-Based Biosensors for the Detection of in Environmental Water Samples.

La Spina R, Antonio D, Desmet C, Valsesia A, Bombera R, Norlen H Sensors (Basel). 2019; 19(21).

PMID: 31717745 PMC: 6864691. DOI: 10.3390/s19214652.

References

Lu W, Arumugam S, Senapati D, Singh A, Arbneshi T, Khan S . Multifunctional oval-shaped gold-nanoparticle-based selective detection of breast cancer cells using simple colorimetric and highly sensitive two-photon scattering assay. ACS Nano. 2010; 4(3):1739-49. PMC: 2844490. DOI: 10.1021/nn901742q. View

Huang Y, Kim D . Dark-field microscopy studies of polarization-dependent plasmonic resonance of single gold nanorods: rainbow nanoparticles. Nanoscale. 2011; 3(8):3228-32. DOI: 10.1039/c1nr10336a. View

Alkilany A, Thompson L, Boulos S, Sisco P, Murphy C . Gold nanorods: their potential for photothermal therapeutics and drug delivery, tempered by the complexity of their biological interactions. Adv Drug Deliv Rev. 2011; 64(2):190-9. DOI: 10.1016/j.addr.2011.03.005. View

Bailey R, Kwong G, Radu C, Witte O, Heath J . DNA-encoded antibody libraries: a unified platform for multiplexed cell sorting and detection of genes and proteins. J Am Chem Soc. 2007; 129(7):1959-67. PMC: 3677962. DOI: 10.1021/ja065930i. View

Nusz G, Marinakos S, Curry A, Dahlin A, Hook F, Wax A . Label-free plasmonic detection of biomolecular binding by a single gold nanorod. Anal Chem. 2008; 80(4):984-9. PMC: 3003601. DOI: 10.1021/ac7017348. View

Yang X, Yu Y, Gao Z . A highly sensitive plasmonic DNA assay based on triangular silver nanoprism etching. ACS Nano. 2014; 8(5):4902-7. DOI: 10.1021/nn5008786. View

Pekcevik I, Poon L, Wang M, Gates B . Tunable loading of single-stranded DNA on gold nanorods through the displacement of polyvinylpyrrolidone. Anal Chem. 2013; 85(20):9960-7. DOI: 10.1021/ac4027737. View

Aslan K, Holley P, Davies L, Lakowicz J, Geddes C . Angular-ratiometric plasmon-resonance based light scattering for bioaffinity sensing. J Am Chem Soc. 2005; 127(34):12115-21. PMC: 6769416. DOI: 10.1021/ja052739k. View

Rissin D, Kan C, Campbell T, Howes S, Fournier D, Song L . Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations. Nat Biotechnol. 2010; 28(6):595-9. PMC: 2919230. DOI: 10.1038/nbt.1641. View

10.

Li G, Zhu L, Wu Z, He Y, Tan H, Sun S . Digital Concentration Readout of DNA by Absolute Quantification of Optically Countable Gold Nanorods. Anal Chem. 2016; 88(22):10994-11000. DOI: 10.1021/acs.analchem.6b02712. View

11.

Taton T, Lu G, Mirkin C . Two-color labeling of oligonucleotide arrays via size-selective scattering of nanoparticle probes. J Am Chem Soc. 2001; 123(21):5164-5. DOI: 10.1021/ja0102639. View

12.

Neely A, Perry C, Varisli B, Singh A, Arbneshi T, Senapati D . Ultrasensitive and highly selective detection of Alzheimer's disease biomarker using two-photon Rayleigh scattering properties of gold nanoparticle. ACS Nano. 2009; 3(9):2834-40. PMC: 2749966. DOI: 10.1021/nn900813b. View

13.

Vollmer F, Arnold S . Whispering-gallery-mode biosensing: label-free detection down to single molecules. Nat Methods. 2008; 5(7):591-6. DOI: 10.1038/nmeth.1221. View

14.

Li T, Wu X, Liu F, Li N . Analytical methods based on the light-scattering of plasmonic nanoparticles at the single particle level with dark-field microscopy imaging. Analyst. 2016; 142(2):248-256. DOI: 10.1039/c6an02384c. View

15.

Darbha G, Ray A, Ray P . Gold nanoparticle-based miniaturized nanomaterial surface energy transfer probe for rapid and ultrasensitive detection of mercury in soil, water, and fish. ACS Nano. 2009; 1(3):208-14. DOI: 10.1021/nn7001954. View

16.

Grasseschi D, Lima F, Nakamura M, Toma H . Hyperspectral dark-field microscopy of gold nanodisks. Micron. 2014; 69:15-20. DOI: 10.1016/j.micron.2014.10.007. View

17.

Duggan D, Bittner M, Chen Y, Meltzer P, Trent J . Expression profiling using cDNA microarrays. Nat Genet. 1999; 21(1 Suppl):10-4. DOI: 10.1038/4434. View

18.

Zhang C, Yeh H, Kuroki M, Wang T . Single-quantum-dot-based DNA nanosensor. Nat Mater. 2005; 4(11):826-31. DOI: 10.1038/nmat1508. View

19.

Jing C, Gu Z, Ying Y, Li D, Zhang L, Long Y . Chrominance to dimension: a real-time method for measuring the size of single gold nanoparticles. Anal Chem. 2012; 84(10):4284-91. DOI: 10.1021/ac203118g. View

20.

Taton T, Mirkin C, LETSINGER R . Scanometric DNA array detection with nanoparticle probes. Science. 2000; 289(5485):1757-60. DOI: 10.1126/science.289.5485.1757. View