Effects of Polysaccharides from Platycodon Grandiflorum on Immunity-Enhancing Activity In Vitro

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2017 Nov 8

PMID 29112148

Citations 16

Authors

Xiaona Zhao

Yuge Wang

Peng Yan

Guodong Cheng

Cheng Wang

Na Geng

Xuepeng Wang

Jianzhu Liu

Affiliations

Soon will be listed here.

Abstract

The study is aimed at investigating the immunoenhancement activity of polysaccharides from polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4⁺ and CD8⁺ T cells were performed. Four different concentrations of PGPSs (PGPS, PGPS, PGPS, and PGPS) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPS groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPS and PGPS were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4⁺ and CD8⁺ T cells. At most time points, PGPS could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4⁺ and CD8⁺ T cells. These results indicate that PGPS enhances the immune functions, suggesting that PGPS could be a potential immunopotentiator for further in vivo and clinical trial experiments.

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