Correlation of Cutaneous Sensitivity and Cytokine Response in Children with Asthma

Overview

Journal Lung India

Specialty Pulmonary Medicine

Date 2017 Nov 4

PMID 29098994

Citations 1

Authors

Meenu Singh

Amit Agarwal

Bishnupada Chatterjee

Anil Chauhan

Rashmi Ranjan Das

Nandini Paul

Affiliations

Soon will be listed here.

Abstract

Background: Food allergy occurs in a significant portion of pediatric asthma. Various cells and their mediators/cytokines play a pivotal role in orchestrating the airway inflammatory response in asthma.

Objective: To study the cutaneous hypersensitivity, Th1, Th2, and Th17 response of pediatric population with asthma and genetic predisposition to atopy, by determining total immunoglobulin E (IgE) level in response to various food allergens.

Materials And Methods: Fifty asthmatic children with a history of worsening symptoms by various food allergens (study group) and twenty healthy children (control group) were included. Food allergy was assessed through skin prick test (SPT) of various food allergens. Total serum IgE level was measured by sandwich ELISA, and T-cell (Th1, Th2, and Th17)-dependent cytokines were measured by flow cytometry.

Results: All 50 asthmatic children in the study group showed SPT positivity against various food allergens (rice = 17; banana, fish and groundnut = 10; wheat = 9; milk and orange = 7; egg = 6; and mango = 4). The average total IgE level in the study group was 316.8 ± 189.8 IU/mL. A significant positive correlation of total IgE with interleukin 17 (IL-17) (r = 0.796; P < 0.0001), IL-13 (r = 0.383; P = 0.01), and IL-4 (r = 0.263; P = 0.043) level was noted. A significant negative correlation of total IgE was noted with interferon gamma (r = -0.5823; P < 0.0001) and IL-10 (r = -0.4474; P < 0.001) level and the duration of breastfeeding (r = -0.31, P = 0.03).

Conclusions: The present study found a positive correlation between total serum IgE level and Th2, Th17 cytokines in a pediatric population with asthma. A significant negative correlation was found between the duration of breastfeeding and the cytokines.

Citing Articles

Food allergy promotes a Th2/Th17 response that drives house dust mite-induced allergic airway inflammation in humanized mice.

Wang B, Hu J, Liu Y, Liu Q, Li D Clin Exp Immunol. 2020; 202(3):300-307.

PMID: 32757273 PMC: 7670146. DOI: 10.1111/cei.13504.

References

Varga E, Nouri-Aria K, Till S, Durham S . Immunomodulatory treatment strategies for allergic diseases. Curr Drug Targets Inflamm Allergy. 2003; 2(1):31-46. DOI: 10.2174/1568010033344507. View

Agrawal S, Townley R . Role of periostin, FENO, IL-13, lebrikzumab, other IL-13 antagonist and dual IL-4/IL-13 antagonist in asthma. Expert Opin Biol Ther. 2013; 14(2):165-81. DOI: 10.1517/14712598.2014.859673. View

Stromnes I, Cerretti L, Liggitt D, Harris R, Goverman J . Differential regulation of central nervous system autoimmunity by T(H)1 and T(H)17 cells. Nat Med. 2008; 14(3):337-42. PMC: 2813727. DOI: 10.1038/nm1715. View

Spencer L, Szela C, Perez S, Kirchhoffer C, Neves J, Radke A . Human eosinophils constitutively express multiple Th1, Th2, and immunoregulatory cytokines that are secreted rapidly and differentially. J Leukoc Biol. 2008; 85(1):117-23. PMC: 2626765. DOI: 10.1189/jlb.0108058. View

Wilson R, Whitehead G, Nakano H, Free M, Kolls J, Cook D . Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness. Am J Respir Crit Care Med. 2009; 180(8):720-30. PMC: 2778149. DOI: 10.1164/rccm.200904-0573OC. View

Hirano K, Hino S, Oshima K, Nadano D, Urisu A, Takaiwa F . Evaluation of allergenic potential for rice seed protein components utilizing a rice proteome database and an allergen database in combination with IgE-binding of recombinant proteins. Biosci Biotechnol Biochem. 2016; 80(3):564-73. DOI: 10.1080/09168451.2015.1116927. View

Kim Y, Hong S, Choi J, Shin T, Moon H, Choi E . Vascular endothelial growth factor is a key mediator in the development of T cell priming and its polarization to type 1 and type 17 T helper cells in the airways. J Immunol. 2009; 183(8):5113-20. PMC: 3385973. DOI: 10.4049/jimmunol.0901566. View

Curtis M, Way S . Interleukin-17 in host defence against bacterial, mycobacterial and fungal pathogens. Immunology. 2009; 126(2):177-85. PMC: 2632692. DOI: 10.1111/j.1365-2567.2008.03017.x. View

Ivanov S, Linden A . Interleukin-17 as a drug target in human disease. Trends Pharmacol Sci. 2009; 30(2):95-103. DOI: 10.1016/j.tips.2008.11.004. View

10.

Tomita K, Lim S, Hanazawa T, Usmani O, Stirling R, Chung K . Attenuated production of intracellular IL-10 and IL-12 in monocytes from patients with severe asthma. Clin Immunol. 2002; 102(3):258-66. DOI: 10.1006/clim.2001.5176. View

11.

Woods R, Thien F, Raven J, Walters E, Abramson M . Prevalence of food allergies in young adults and their relationship to asthma, nasal allergies, and eczema. Ann Allergy Asthma Immunol. 2002; 88(2):183-9. DOI: 10.1016/S1081-1206(10)61994-1. View

12.

Minniti F, Comberiati P, Munblit D, Piacentini G, Antoniazzi E, Zanoni L . Breast-milk characteristics protecting against allergy. Endocr Metab Immune Disord Drug Targets. 2014; 14(1):9-15. DOI: 10.2174/1871530314666140121145045. View

13.

Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N . IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice. Am J Respir Crit Care Med. 2008; 178(10):1023-32. DOI: 10.1164/rccm.200801-086OC. View

14.

Usui T, Nishikomori R, Kitani A, Strober W . GATA-3 suppresses Th1 development by downregulation of Stat4 and not through effects on IL-12Rbeta2 chain or T-bet. Immunity. 2003; 18(3):415-28. DOI: 10.1016/s1074-7613(03)00057-8. View

15.

Grunig G . IL-13 and adenosine: partners in a molecular dance?. J Clin Invest. 2003; 112(3):329-31. PMC: 166304. DOI: 10.1172/JCI19392. View

16.

Linden A, Laan M, Anderson G . Neutrophils, interleukin-17A and lung disease. Eur Respir J. 2005; 25(1):159-72. DOI: 10.1183/09031936.04.00032904. View

17.

Chernyshov P . B7-2/CD28 costimulatory pathway in children with atopic dermatitis and its connection with immunoglobulin E, intracellular interleukin-4 and interferon-gamma production by T cells during a 1-month follow-up. J Eur Acad Dermatol Venereol. 2009; 23(6):656-9. DOI: 10.1111/j.1468-3083.2009.03133.x. View

18.

Holgate S, Polosa R . Treatment strategies for allergy and asthma. Nat Rev Immunol. 2008; 8(3):218-30. DOI: 10.1038/nri2262. View

19.

Raedler D, Ballenberger N, Klucker E, Bock A, Otto R, Prazeres da Costa O . Identification of novel immune phenotypes for allergic and nonallergic childhood asthma. J Allergy Clin Immunol. 2014; 135(1):81-91. DOI: 10.1016/j.jaci.2014.07.046. View

20.

Park S, Lee Y . Interleukin-17 regulation: an attractive therapeutic approach for asthma. Respir Res. 2010; 11:78. PMC: 2894770. DOI: 10.1186/1465-9921-11-78. View