Cellular V-ATPase is Required for Virion Assembly Compartment Formation in Human Cytomegalovirus Infection

Overview

Journal Open Biol

Specialties Biology
Cell Biology
Molecular Biology

Date 2017 Nov 3

PMID 29093211

Citations 11

Authors

Jonathan Pavelin

Dominique McCormick

Stephen Chiweshe

Saranya Ramachandran

Yao-Tang Lin

Finn Grey

Affiliations

Soon will be listed here.

Abstract

Successful generation of virions from infected cells is a complex process requiring orchestrated regulation of host and viral genes. Cells infected with human cytomegalovirus (HCMV) undergo a dramatic reorganization of membrane organelles resulting in the formation of the virion assembly compartment, a process that is not fully understood. Here we show that acidification of vacuoles by the cellular v-ATPase is a crucial step in the formation of the virion assembly compartment and disruption of acidification results in mis-localization of virion components and a profound reduction in infectious virus levels. In addition, knockdown of ATP6V0C blocks the increase in nuclear size, normally associated with HCMV infection. Inhibition of the v-ATPase does not affect intracellular levels of viral DNA synthesis or gene expression, consistent with a defect in assembly and egress. These studies identify a novel host factor involved in virion production and a potential target for antiviral therapy.

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