Effect of Hyperthermia on Calbindin-D 28k Immunoreactivity in the Hippocampal Formation Following Transient Global Cerebral Ischemia in Gerbils

Overview

Journal Neural Regen Res

Date 2017 Nov 2

PMID 29089991

Citations 4

Authors

Jae-Chul Lee

Jeong-Hwi Cho

Tae-Kyeong Lee

In Hye Kim

Moo-Ho Won

Geum-Sil Cho

Bich-Na Shin

In Koo Hwang

Joon Ha Park

Ji Hyeon Ahn

Il Jun Kang

Young Joo Lee

Yang Hee Kim

Affiliations

Soon will be listed here.

Abstract

Calbindin D-28K (CB), a Ca-binding protein, maintains Ca homeostasis and protects neurons against various insults. Hyperthermia can exacerbate brain damage produced by ischemic insults. However, little is reported about the role of CB in the brain under hyperthermic condition during ischemic insults. We investigated the effects of transient global cerebral ischemia on CB immunoreactivity as well as neuronal damage in the hippocampal formation under hyperthermic condition using immunohistochemistry for neuronal nuclei (NeuN) and CB, and Fluoro-Jade B histofluorescence staining in gerbils. Hyperthermia (39.5 ± 0.2°C) was induced for 30 minutes before and during transient ischemia. Hyperthermic ischemia resulted in neuronal damage/death in the pyramidal layer of CA1-3 area and in the polymorphic layer of the dentate gyrus at 1, 2, 5 days after ischemia. In addition, hyperthermic ischemia significantly decreaced CB immunoreactivity in damaged or dying neurons at 1, 2, 5 days after ischemia. In brief, hyperthermic condition produced more extensive and severer neuronal damage/death, and reduced CB immunoreactivity in the hippocampus following transient global cerebral ischemia. Present findings indicate that the degree of reduced CB immunoreactivity might be related with various neuronal damage/death overtime and corresponding areas after ischemic insults.

Citing Articles

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References

Li M, Inoue K, Si H, Xiong Z . Calcium-permeable ion channels involved in glutamate receptor-independent ischemic brain injury. Acta Pharmacol Sin. 2011; 32(6):734-40. PMC: 3395229. DOI: 10.1038/aps.2011.47. View

Schmued L, Hopkins K . Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration. Brain Res. 2000; 874(2):123-30. DOI: 10.1016/s0006-8993(00)02513-0. View

Heizmann C, Braun K . Changes in Ca(2+)-binding proteins in human neurodegenerative disorders. Trends Neurosci. 1992; 15(7):259-64. DOI: 10.1016/0166-2236(92)90067-i. View

Bano D, Nicotera P . Ca2+ signals and neuronal death in brain ischemia. Stroke. 2007; 38(2 Suppl):674-6. DOI: 10.1161/01.STR.0000256294.46009.29. View

Dietrich W, Busto R, Valdes I, Loor Y . Effects of normothermic versus mild hyperthermic forebrain ischemia in rats. Stroke. 1990; 21(9):1318-25. DOI: 10.1161/01.str.21.9.1318. View

Wang Y, Lim L, Levi C, Heller R, Fisher J . Influence of admission body temperature on stroke mortality. Stroke. 2000; 31(2):404-9. DOI: 10.1161/01.str.31.2.404. View

GREENE J, Kerkhoff J, Guiver L, Totterdell S . Structural and functional abnormalities of the hippocampal formation in rats with environmentally induced reductions in prepulse inhibition of acoustic startle. Neuroscience. 2001; 103(2):315-23. DOI: 10.1016/s0306-4522(00)00560-1. View

Schmidt-Kastner R, Freund T . Selective vulnerability of the hippocampus in brain ischemia. Neuroscience. 1991; 40(3):599-636. DOI: 10.1016/0306-4522(91)90001-5. View

Klapstein G, Vietla S, Lieberman D, Gray P, Airaksinen M, Thoenen H . Calbindin-D28k fails to protect hippocampal neurons against ischemia in spite of its cytoplasmic calcium buffering properties: evidence from calbindin-D28k knockout mice. Neuroscience. 1998; 85(2):361-73. DOI: 10.1016/s0306-4522(97)00632-5. View

10.

Lee J, Tae H, Kim I, Cho J, Lee T, Park J . Roles of HIF-1α, VEGF, and NF-κB in Ischemic Preconditioning-Mediated Neuroprotection of Hippocampal CA1 Pyramidal Neurons Against a Subsequent Transient Cerebral Ischemia. Mol Neurobiol. 2016; 54(9):6984-6998. DOI: 10.1007/s12035-016-0219-2. View

11.

Corbett D, Thornhill J . Temperature modulation (hypothermic and hyperthermic conditions) and its influence on histological and behavioral outcomes following cerebral ischemia. Brain Pathol. 2000; 10(1):145-52. PMC: 8098623. DOI: 10.1111/j.1750-3639.2000.tb00251.x. View

12.

Seo W, Yu S, Kim J, Park K, Koh S . The impact of hyperthermia and infection on acute ischemic stroke patients in the intensive care unit. Neurocrit Care. 2008; 9(2):183-8. DOI: 10.1007/s12028-008-9056-0. View

13.

Busto R, Globus M, Neary J, Ginsberg M . Regional alterations of protein kinase C activity following transient cerebral ischemia: effects of intraischemic brain temperature modulation. J Neurochem. 1994; 63(3):1095-103. DOI: 10.1046/j.1471-4159.1994.63031095.x. View

14.

Minamisawa H, Smith M, Siesjo B . The effect of mild hyperthermia and hypothermia on brain damage following 5, 10, and 15 minutes of forebrain ischemia. Ann Neurol. 1990; 28(1):26-33. DOI: 10.1002/ana.410280107. View

15.

Lee Y, Yan B, Park J, Ahn J, Kim I, Lee J . Differences of calcium binding proteins immunoreactivities in the young hippocampal CA1 region from the adult following transient ischemic damage. J Neurol Sci. 2013; 326(1-2):40-7. DOI: 10.1016/j.jns.2012.12.026. View

16.

Kim M, Cho J, Cho J, Park J, Ahn J, Tae H . Impact of hyperthermia before and during ischemia-reperfusion on neuronal damage and gliosis in the gerbil hippocampus induced by transient cerebral ischemia. J Neurol Sci. 2014; 348(1-2):101-10. DOI: 10.1016/j.jns.2014.11.015. View

17.

Mattson M, Rychlik B, Chu C, Christakos S . Evidence for calcium-reducing and excito-protective roles for the calcium-binding protein calbindin-D28k in cultured hippocampal neurons. Neuron. 1991; 6(1):41-51. DOI: 10.1016/0896-6273(91)90120-o. View

18.

Dietrich W, Halley M, Valdes I, Busto R . Interrelationships between increased vascular permeability and acute neuronal damage following temperature-controlled brain ischemia in rats. Acta Neuropathol. 1991; 81(6):615-25. DOI: 10.1007/BF00296371. View

19.

Bae E, Chen B, Shin B, Cho J, Kim I, Park J . Comparison of immunoreactivities of calbindin-D28k, calretinin and parvalbumin in the striatum between young, adult and aged mice, rats and gerbils. Neurochem Res. 2015; 40(4):864-72. DOI: 10.1007/s11064-015-1537-x. View

20.

Kim Y, Busto R, Dietrich W, Kraydieh S, Ginsberg M . Delayed postischemic hyperthermia in awake rats worsens the histopathological outcome of transient focal cerebral ischemia. Stroke. 1996; 27(12):2274-80; discussion 2281. DOI: 10.1161/01.str.27.12.2274. View