Protective Role of LRRC3B in Preventing Breast Cancer Metastasis and Recurrence Post-bupivacaine

Overview

Journal Oncol Lett

Specialty Oncology

Date 2017 Nov 1

PMID 29085514

Citations 2

Authors

Gong-Sheng Li

Gao-Yin Kong

Yi Zou

Affiliations

Soon will be listed here.

Abstract

The present study aimed to investigate the potential effect of leucine-rich repeat containing 3B (LRRC3B) with respect to the inhibition of breast cancer recurrence and metastasis post-anesthesia. The mRNA expression of LRRC3B in breast MDA-MB-231 and MCF-7 cell lines was detected using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. The effect of bupivacaine on breast cancer cell invasion was analyzed using a Matrigel assay. LRRC3B specific small interfering (si)RNA was constructed and transfected into breast cancer cells using Lipofectamine 2000 reagent. The influence of bupivacaine on LRRC3B expression was measured based on RT-qPCR. Additionally, the effect of LRRC3B silencing on the invasion of breast cancer cells treated with bupivacaine was analyzed. Compared with the control, LRRC3B expression significantly increased in MDA-MB-231 and in MCF-7 cells as the length of time increased (P<0.05), but the expression of the gene significantly declined in 2 types of cancer cell when the cells were transfected with siRNA-LRRC3B plasma (P<0.05). The administration of 50 µg/ml bupivacaine promoted maximum breast cancer cell invasion, and suppressed LRRC3B mRNA expression in cells. However, when LRRC3B was silenced in cancer cells, 20 µg/ml bupivacaine significantly promoted cancer cell invasion, indicating that bupivacaine suppresses the expression of LRRC3B and promotes cell invasion. The present study suggested that LRRC3B serves a protective role in preventing bupivacaine-induced breast cancer recurrence and metastasis.

Citing Articles

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References

Chow S, Kao C, Liu Y, Cheng M, Yang Y, Huang Y . Resveratrol induced ER expansion and ER caspase-mediated apoptosis in human nasopharyngeal carcinoma cells. Apoptosis. 2013; 19(3):527-41. DOI: 10.1007/s10495-013-0945-0. View

Mariotto A, Noone A, Howlader N, Cho H, Keel G, Garshell J . Cancer survival: an overview of measures, uses, and interpretation. J Natl Cancer Inst Monogr. 2014; 2014(49):145-86. PMC: 4829054. DOI: 10.1093/jncimonographs/lgu024. View

Haraldson K, Kashuba V, Dmitriev A, Senchenko V, Kudryavtseva A, Pavlova T . LRRC3B gene is frequently epigenetically inactivated in several epithelial malignancies and inhibits cell growth and replication. Biochimie. 2012; 94(5):1151-7. DOI: 10.1016/j.biochi.2012.01.019. View

Exadaktylos A, Buggy D, Moriarty D, Mascha E, Sessler D . Can anesthetic technique for primary breast cancer surgery affect recurrence or metastasis?. Anesthesiology. 2006; 105(4):660-4. PMC: 1615712. DOI: 10.1097/00000542-200610000-00008. View

Li Y, Han N, Yin T, Huang L, Liu S, Liu D . Lentivirus-mediated Nox4 shRNA invasion and angiogenesis and enhances radiosensitivity in human glioblastoma. Oxid Med Cell Longev. 2014; 2014:581732. PMC: 4020159. DOI: 10.1155/2014/581732. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Sessler D, Ben-Eliyahu S, Mascha E, Parat M, Buggy D . Can regional analgesia reduce the risk of recurrence after breast cancer? Methodology of a multicenter randomized trial. Contemp Clin Trials. 2008; 29(4):517-26. DOI: 10.1016/j.cct.2008.01.002. View

Marwick P, Levin A, Coetzee A . Recurrence of cardiotoxicity after lipid rescue from bupivacaine-induced cardiac arrest. Anesth Analg. 2009; 108(4):1344-6. DOI: 10.1213/ane.0b013e3181979e17. View

De Oliveira Jr G, Ahmad S, Schink J, Singh D, Fitzgerald P, McCarthy R . Intraoperative neuraxial anesthesia but not postoperative neuraxial analgesia is associated with increased relapse-free survival in ovarian cancer patients after primary cytoreductive surgery. Reg Anesth Pain Med. 2011; 36(3):271-7. DOI: 10.1097/AAP.0b013e318217aada. View

10.

Lacassie H, Cartagena J, Branes J, Assel M, Echevarria G . The relationship between neuraxial anesthesia and advanced ovarian cancer-related outcomes in the Chilean population. Anesth Analg. 2013; 117(3):653-660. DOI: 10.1213/ANE.0b013e3182a07046. View

11.

Tian X, Zhang Y, Sun D, Zhao S, Xiong H, Fang J . Epigenetic silencing of LRRC3B in colorectal cancer. Scand J Gastroenterol. 2008; 44(1):79-84. DOI: 10.1080/00365520802400834. View

12.

de Leon-Casasola O, Parker B, Lema M, Harrison P, Massey J . Postoperative epidural bupivacaine-morphine therapy. Experience with 4,227 surgical cancer patients. Anesthesiology. 1994; 81(2):368-75. DOI: 10.1097/00000542-199408000-00015. View

13.

Jorgensen J, Juliusson B, Henriksen K, Hansen C, Knudsen S, Petersen T . Identification of novel genes regulated in the developing human ventral mesencephalon. Exp Neurol. 2006; 198(2):427-37. DOI: 10.1016/j.expneurol.2005.12.023. View

14.

Wang Y, Klijn J, Zhang Y, Sieuwerts A, Look M, Yang F . Gene-expression profiles to predict distant metastasis of lymph-node-negative primary breast cancer. Lancet. 2005; 365(9460):671-9. DOI: 10.1016/S0140-6736(05)17947-1. View

15.

Liang Z, Wu H, Reddy S, Zhu A, Wang S, Blevins D . Blockade of invasion and metastasis of breast cancer cells via targeting CXCR4 with an artificial microRNA. Biochem Biophys Res Commun. 2007; 363(3):542-6. DOI: 10.1016/j.bbrc.2007.09.007. View

16.

Deegan C, Murray D, Doran P, Moriarty D, Sessler D, Mascha E . Anesthetic technique and the cytokine and matrix metalloproteinase response to primary breast cancer surgery. Reg Anesth Pain Med. 2010; 35(6):490-5. DOI: 10.1097/AAP.0b013e3181ef4d05. View

17.

Al-Sahaf O, Wang J, Browne T, Cotter T, Redmond H . Surgical injury enhances the expression of genes that mediate breast cancer metastasis to the lung. Ann Surg. 2010; 252(6):1037-43. DOI: 10.1097/SLA.0b013e3181efc635. View

18.

You Y, Yang W, Qin X, Wang F, Li H, Lin C . ECRG4 acts as a tumor suppressor and as a determinant of chemotherapy resistance in human nasopharyngeal carcinoma. Cell Oncol (Dordr). 2015; 38(3):205-14. DOI: 10.1007/s13402-015-0223-y. View

19.

Mohamed S, Fares K, Mohamed A, Alieldin N . Dexmedetomidine as an adjunctive analgesic with bupivacaine in paravertebral analgesia for breast cancer surgery. Pain Physician. 2014; 17(5):E589-98. View

20.

Senkus E, Kyriakides S, Ohno S, Penault-Llorca F, Poortmans P, Rutgers E . Primary breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol. 2015; 26 Suppl 5:v8-30. DOI: 10.1093/annonc/mdv298. View