Cellular-level Real-time Pharmacokinetic Imaging of Free-form and Liposomal Indocyanine Green in Liver

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2017 Oct 31

PMID 29082096

Citations 14

Authors

Yoonha Hwang

Hwanjun Yoon

Kibaek Choe

Jinhyo Ahn

Jik Han Jung

Ji-Ho Park

Pilhan Kim

Affiliations

Soon will be listed here.

Abstract

Indocyanine green (ICG) is a near-infrared fluorophore approved for human use which has been widely used for various clinical applications. Despite the well-established clinical usage, our understanding about the microscopic pharmacokinetics of systemically administered ICG has been relatively limited. In this work, we successfully visualized real-time pharmacokinetic dynamics of the intravenously injected free-form and liposomal ICG in cellular resolution by utilizing a custom-built video-rate near infrared laser-scanning confocal microscopy system. Initial perfusion and clearance from blood stream, diffusion into perisinusoidal space, and subsequent absorption into hepatocyte were directly visualized . The quantification analysis utilizing the real-time image sequences revealed distinct dynamic pharmacokinetic behavior of free-form and liposomal ICG.

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