Binding of Harmine Derivatives to DNA: A Spectroscopic Investigation

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2017 Oct 28

PMID 29077046

Citations 3

Authors

Bruno Pagano

Marco Caterino

Rosanna Filosa

Concetta Giancola

Affiliations

Soon will be listed here.

Abstract

Harmine belongs to a group of β-carboline alkaloids endowed with antitumor properties. Harmine and its derivatives are thought to bind to DNA and interfere with topoisomerase activities. We investigated the base-dependent binding of harmine, and three of its synthetic anticancer-active derivatives to the genomic DNA from calf thymus and two synthetic 20-mer double helices, the poly(dG-dC)·poly(dG-dC) and the poly(dA-dT)·poly(dA-dT), by means of UV-Vis and circular dichroism (CD) spectroscopies. The data show that the DNA binding and stabilising properties of the investigated derivatives are base pair-dependent. These results could be used as a guide to design and develop further bioactive analogues.

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