Single Chain Antibody Fragment Against Venom from the Snake Daboia Russelii Formosensis

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2017 Oct 28

PMID 29076991

Citations 11

Authors

Chi-Hsin Lee

Yu-Ching Lee

Yueh-Lun Lee

Sy-Jye Leu

Liang-Tzung Lin

Chi-Ching Chen

Jen-Ron Chiang

Pharaoh Fellow Mwale

Bor-Yu Tsai

Ching-Sheng Hung

Yi-Yuan Yang

Affiliations

Soon will be listed here.

Abstract

Russell's vipers containing hemotoxic and neurotoxic venom commonly cause snake envenomation. Horse-derived antivenom is a specific antidote, but its production is expensive and has side effects. Developing a cost-effective and more tolerable therapeutic strategy is favorable. In this study, using glutaraldehyde-attenuated (DRF) venom proteins to immunize chickens, polyclonal yolk-immunoglobulin (IgY) antibodies were generated and showed a specific binding affinity. Phage display technology was used to generate two antibody libraries of single-chain variable fragments (scFvs) containing 3.4 × 10⁷ and 5.5 × 10⁷ transformants, respectively. Phage-based ELISA indicated that specific clones were enriched after bio-panning. The nucleotide sequences of scFv-expressing clones were analyzed and classified into six groups in the short linker and four groups in the long linker. These scFv antibodies specifically bound to DRF proteins, but not other venom proteins. Mass spectrometric data suggested that these scFv antibodies may recognize phospholipase A2 RV-4 or RV-7. In vivo studies showed that anti-DRF IgY exhibited complete protective effects and mixed scFv antibodies increased the survival rate and time of mice challenged with a lethal dose of DRF proteins. These antibodies can be potentially applied in a rapid diagnostic method or for treatment in the future.

Citing Articles

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