Toxicological Assessment and UV/TiO-Based Induced Degradation Profile of Reactive Black 5 Dye

Overview

Journal Environ Manage

Specialties Environmental Health
Toxicology

Date 2017 Oct 27

PMID 29071551

Citations 6

Authors

Muhammad Bilal

Tahir Rasheed

Hafiz M N Iqbal

Hongbo Hu

Wei Wang

Xuehong Zhang

Affiliations

Soon will be listed here.

Abstract

In this study, the toxicological and degradation profile of Reactive Black 5 (RB5) dye was evaluated using a UV/TiO-based degradation system. Fourier transform infrared spectroscopy (FT-IR), thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS) techniques were used to evaluate the degradation level of RB5. The UV-Vis spectral analysis revealed the disappearance of peak intensity at 599 nm (λmax). The FT-IR spectrum of UV/TiO treated dye sample manifest appearance of new peaks mainly because of the degraded product and/or disappearance of some characteristics peaks which were present in the untreated spectrum. The HPLC profile verified the RB5 degradation subject to the formation of metabolites at different retention times. A stable color removal higher than 96% with COD removal in the range of 74-82.3% was noted at all evaluated dye concentrations. The tentative degradation pathway of RB5 is proposed following a careful analysis of the intermediates identified by UPLC-MS. Toxicity profile of untreated and degraded dye samples was monitored using three types of human cell lines via MTT assay and acute toxicity testing with Artemia salina. In conclusion, the UV/TiO-based degradation system could be effectively employed for the remediation of textile wastewater comprising a high concentration of reactive dyes.

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References

Plum A, Braun G, Rehorek A . Process monitoring of anaerobic azo dye degradation by high-performance liquid chromatography-diode array detection continuously coupled to membrane filtration sampling modules. J Chromatogr A. 2003; 987(1-2):395-402. DOI: 10.1016/s0021-9673(02)01906-4. View

Gahlout M, Gupte S, Gupte A . Optimization of culture condition for enhanced decolorization and degradation of azo dye reactive violet 1 with concomitant production of ligninolytic enzymes by Ganoderma cupreum AG-1. 3 Biotech. 2017; 3(2):143-152. PMC: 3597137. DOI: 10.1007/s13205-012-0079-z. View

Fischer D, Li Y, Ahlemeyer B, Krieglstein J, Kissel T . In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. Biomaterials. 2003; 24(7):1121-31. DOI: 10.1016/s0142-9612(02)00445-3. View

Neoh C, Lam C, Lim C, Yahya A, Bay H, Ibrahim Z . Biodecolorization of recalcitrant dye as the sole sourceof nutrition using Curvularia clavata NZ2 and decolorization ability of its crude enzymes. Environ Sci Pollut Res Int. 2015; 22(15):11669-78. DOI: 10.1007/s11356-015-4436-4. View

Abadulla E, Tzanov T, Costa S, Robra K, Cavaco-Paulo A, Gubitz G . Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl Environ Microbiol. 2000; 66(8):3357-62. PMC: 92155. DOI: 10.1128/AEM.66.8.3357-3362.2000. View

Lade H, Govindwar S, Paul D . Mineralization and Detoxification of the Carcinogenic Azo Dye Congo Red and Real Textile Effluent by a Polyurethane Foam Immobilized Microbial Consortium in an Upflow Column Bioreactor. Int J Environ Res Public Health. 2015; 12(6):6894-918. PMC: 4483738. DOI: 10.3390/ijerph120606894. View

Robinson T, McMullan G, Marchant R, Nigam P . Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative. Bioresour Technol. 2001; 77(3):247-55. DOI: 10.1016/s0960-8524(00)00080-8. View

Jadhav J, Phugare S, Dhanve R, Jadhav S . Rapid biodegradation and decolorization of Direct Orange 39 (Orange TGLL) by an isolated bacterium Pseudomonas aeruginosa strain BCH. Biodegradation. 2009; 21(3):453-63. DOI: 10.1007/s10532-009-9315-6. View

Elmorsi T, Riyad Y, Mohamed Z, Abd El Bary H . Decolorization of Mordant red 73 azo dye in water using H2O2/UV and photo-Fenton treatment. J Hazard Mater. 2009; 174(1-3):352-8. DOI: 10.1016/j.jhazmat.2009.09.057. View

10.

Bilal M, Asgher M . Dye decolorization and detoxification potential of Ca-alginate beads immobilized manganese peroxidase. BMC Biotechnol. 2015; 15:111. PMC: 4676175. DOI: 10.1186/s12896-015-0227-8. View

11.

Sohrabi M, Ghavami M . Photocatalytic degradation of Direct Red 23 dye using UV/TiO2: Effect of operational parameters. J Hazard Mater. 2007; 153(3):1235-9. DOI: 10.1016/j.jhazmat.2007.09.114. View

12.

Bilal M, Asgher M, Iqbal H, Hu H, Wang W, Zhang X . Bio-catalytic performance and dye-based industrial pollutants degradation potential of agarose-immobilized MnP using a Packed Bed Reactor System. Int J Biol Macromol. 2017; 102:582-590. DOI: 10.1016/j.ijbiomac.2017.04.065. View

13.

Matthews R . Artemia salina as a test organism for measuring superoxide-mediated toxicity. Free Radic Biol Med. 1995; 18(5):919-22. DOI: 10.1016/0891-5849(94)00205-x. View

14.

Oturkar C, Patole M, Gawai K, Madamwar D . Enzyme based cleavage strategy of Bacillus lentus BI377 in response to metabolism of azoic recalcitrant. Bioresour Technol. 2013; 130:360-5. DOI: 10.1016/j.biortech.2012.12.019. View

15.

Bilal M, Iqbal H, Hu H, Wang W, Zhang X . Enhanced bio-catalytic performance and dye degradation potential of chitosan-encapsulated horseradish peroxidase in a packed bed reactor system. Sci Total Environ. 2016; 575:1352-1360. DOI: 10.1016/j.scitotenv.2016.09.215. View

16.

Asgher M, Shah S, Iqbal H . Statistical Correlation between Ligninolytic Enzymes Secretion and Remazol Brilliant Yellow-3GL Dye Degradation Potential of Trametes versicolor IBL-04. Water Environ Res. 2016; 88(4):338-45. DOI: 10.2175/106143016X14504669768011. View

17.

Bilal M, Iqbal H, Shah S, Hu H, Wang W, Zhang X . Horseradish peroxidase-assisted approach to decolorize and detoxify dye pollutants in a packed bed bioreactor. J Environ Manage. 2016; 183(Pt 3):836-842. DOI: 10.1016/j.jenvman.2016.09.040. View

18.

Arun Prasad A, Rao K . Aerobic biodegradation of Azo dye by Bacillus cohnii MTCC 3616; an obligately alkaliphilic bacterium and toxicity evaluation of metabolites by different bioassay systems. Appl Microbiol Biotechnol. 2012; 97(16):7469-81. DOI: 10.1007/s00253-012-4492-3. View

19.

Chatha S, Asgher M, Iqbal H . Enzyme-based solutions for textile processing and dye contaminant biodegradation-a review. Environ Sci Pollut Res Int. 2017; 24(16):14005-14018. DOI: 10.1007/s11356-017-8998-1. View

20.

Bilal M, Asgher M, Iqbal H, Hu H, Zhang X . Bio-based degradation of emerging endocrine-disrupting and dye-based pollutants using cross-linked enzyme aggregates. Environ Sci Pollut Res Int. 2017; 24(8):7035-7041. DOI: 10.1007/s11356-017-8369-y. View