Involvement of DNA Mismatch Repair in the Maintenance of Heterochromatic DNA Stability in Saccharomyces Cerevisiae

Overview

Journal PLoS Genet

Specialty Genetics

Date 2017 Oct 26

PMID 29069084

Citations 3

Authors

Basanta K Dahal

Lyudmila Y Kadyrova

Kristin R Delfino

Igor B Rogozin

Vaibhavi Gujar

Kirill S Lobachev

Farid A Kadyrov

Affiliations

Soon will be listed here.

Abstract

Heterochromatin contains a significant part of nuclear DNA. Little is known about the mechanisms that govern heterochromatic DNA stability. We show here that in the yeast Saccharomyces cerevisiae (i) DNA mismatch repair (MMR) is required for the maintenance of heterochromatic DNA stability, (ii) MutLα (Mlh1-Pms1 heterodimer), MutSα (Msh2-Msh6 heterodimer), MutSβ (Msh2-Msh3 heterodimer), and Exo1 are involved in MMR at heterochromatin, (iii) Exo1-independent MMR at heterochromatin frequently leads to the formation of Pol ζ-dependent mutations, (iv) MMR cooperates with the proofreading activity of Pol ε and the histone acetyltransferase Rtt109 in the maintenance of heterochromatic DNA stability, (v) repair of base-base mismatches at heterochromatin is less efficient than repair of base-base mismatches at euchromatin, and (vi) the efficiency of repair of 1-nt insertion/deletion loops at heterochromatin is similar to the efficiency of repair of 1-nt insertion/deletion loops at euchromatin.

Citing Articles

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