Combination of Interleukin-10 Gene Promoter Polymorphisms with HLA-DRB1*15 Allele is Associated with Multiple Sclerosis

Overview

Journal Indian J Med Res

Specialty General Medicine

Date 2017 Oct 26

PMID 29067976

Citations 3

Authors

Majid Shahbazi

Javad Sadeghi Allah Abadi

Danial Roshandel

Maryam Koochaki

Hosein Amiri

Rahim Kohansal

Seied Mohammad Baghbanian

Mahdi Zamani

Affiliations

Soon will be listed here.

Abstract

Background & Objectives: Multiple sclerosis (MS) is common in some ethnic groups. Interleukin-10 (IL-10) is a potent anti-inflammatory and immunosuppressive cytokine that may be an important regulator in MS disease pathogenesis. IL-10 promoter includes several single nucleotide polymorphisms and the level of IL-10 expression is related to these polymorphisms. Furthermore, loci within the histocompatibility regions are responsible for susceptibility to MS. The aim of this study was to investigate the association of IL-10 gene promoter polymorphisms and HLA-DRB1*15 allele frequencies with MS susceptibility in an Iranian population.

Methods: In this study 336 MS patients and 454 healthy controls were included. Genomic DNA was purified from peripheral blood samples by a standard protocol. Genotyping was performed by the sequence-specific primer polymerase chain reaction method.

Results: IL-10 -1082 G/G and IL-10 -819 C/C genotypes were more frequent in MS patients than healthy individuals. DRB1*15 allele showed a higher frequency among MS patients compared to controls.

Interpretation & Conclusions: The IL-10 and HLA-DRB1*15 polymorphisms were associated with the susceptibility to MS in Iranian patients. Our results suggest that gene-gene interaction of IL-10 polymorphisms and HLA-DRB1*15 alleles may be important factors in the development of MS.

Citing Articles

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Intestinal mucosa-derived DNA methylation signatures in the penetrating intestinal mucosal lesions of Crohn's disease.

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