Towards Better Understanding of Pea Seed Dormancy Using Laser Desorption/Ionization Mass Spectrometry

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2017 Oct 26

PMID 29065445

Citations 7

Authors

Monika Cechova

Marketa Valkova

Iveta Hradilova

Anna Janska

Ales Soukup

Petr Smykal

Petr Bednar

Affiliations

Soon will be listed here.

Abstract

Seed coats of six pea genotypes contrasting in dormancy were studied by laser desorption/ionization mass spectrometry (LDI-MS). Multivariate statistical analysis discriminated dormant and non-dormant seeds in mature dry state. Separation between dormant and non-dormant types was observed despite important markers of particular dormant genotypes differ from each other. Normalized signals of long-chain hydroxylated fatty acids (HLFA) in dormant JI64 genotype seed coats were significantly higher than in other genotypes. These compounds seem to be important markers likely influencing JI64 seed imbibition and germination. HLFA importance was supported by study of recombinant inbred lines (JI64xJI92) contrasting in dormancy but similar in other seed properties. Furthemore HLFA distribution in seed coat was studied by mass spectrometry imaging. HLFA contents in strophiole and hilum are significantly lower compared to other parts indicating their role in water uptake. Results from LDI-MS experiments are useful in understanding (physical) dormancy (first phases of germination) mechanism and properties related to food processing technologies (e.g., seed treatment by cooking).

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References

Bentsink L, Koornneef M . Seed dormancy and germination. Arabidopsis Book. 2012; 6:e0119. PMC: 3243337. DOI: 10.1199/tab.0119. View

Shroff R, Muck A, Svatos A . Analysis of low molecular weight acids by negative mode matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2007; 21(20):3295-300. DOI: 10.1002/rcm.3216. View

Chai M, Zhou C, Molina I, Fu C, Nakashima J, Li G . A class II KNOX gene, KNOX4, controls seed physical dormancy. Proc Natl Acad Sci U S A. 2016; 113(25):6997-7002. PMC: 4922145. DOI: 10.1073/pnas.1601256113. View

North H, Casey R, Domoney C . Inheritance and mapping of seed lipoxygenase polypeptides in Pisum. Theor Appl Genet. 2013; 77(6):805-8. DOI: 10.1007/BF00268330. View

Dam S, Laursen B, Ornfelt J, Jochimsen B, Staerfeldt H, Friis C . The proteome of seed development in the model legume Lotus japonicus. Plant Physiol. 2009; 149(3):1325-40. PMC: 2649391. DOI: 10.1104/pp.108.133405. View

Hradilova I, Trneny O, Valkova M, Cechova M, Janska A, Prokesova L . A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea ( sp.). Front Plant Sci. 2017; 8:542. PMC: 5404241. DOI: 10.3389/fpls.2017.00542. View

Worley B, Powers R . Multivariate Analysis in Metabolomics. Curr Metabolomics. 2015; 1(1):92-107. PMC: 4465187. DOI: 10.2174/2213235X11301010092. View

Smykal P, Vernoud V, Blair M, Soukup A, Thompson R . The role of the testa during development and in establishment of dormancy of the legume seed. Front Plant Sci. 2014; 5:351. PMC: 4102250. DOI: 10.3389/fpls.2014.00351. View

Pirkl A, Meier M, Popkova Y, Letzel M, Schnapp A, Schiller J . Analysis of free fatty acids by ultraviolet laser desorption ionization mass spectrometry using insect wings as hydrophobic sample substrates. Anal Chem. 2014; 86(21):10763-71. DOI: 10.1021/ac5020047. View

10.

Metzger J . Role of Endogenous Plant Growth Regulators in Seed Dormancy of Avena fatua: II. Gibberellins. Plant Physiol. 1983; 73(3):791-5. PMC: 1066550. DOI: 10.1104/pp.73.3.791. View

11.

Lesiak A, Cody R, Dane A, Musah R . Plant seed species identification from chemical fingerprints: a high-throughput application of direct analysis in real time mass spectrometry. Anal Chem. 2015; 87(17):8748-57. DOI: 10.1021/acs.analchem.5b01611. View

12.

MacGregor D, Kendall S, Florance H, Fedi F, Moore K, Paszkiewicz K . Seed production temperature regulation of primary dormancy occurs through control of seed coat phenylpropanoid metabolism. New Phytol. 2014; 205(2):642-52. DOI: 10.1111/nph.13090. View

13.

Rodriguez-Gacio M, Matilla-Vazquez M, Matilla A . Seed dormancy and ABA signaling: the breakthrough goes on. Plant Signal Behav. 2009; 4(11):1035 - 49. PMC: 2819511. DOI: 10.4161/psb.4.11.9902. View

14.

Jiang J, Shao Y, Li A, Zhang Y, Wei C, Wang Y . FT-IR and NMR study of seed coat dissected from different colored progenies of Brassica napus-Sinapis alba hybrids. J Sci Food Agric. 2012; 93(8):1898-902. DOI: 10.1002/jsfa.5986. View

15.

Budimir N, Blais J, Fournier F, Tabet J . The use of desorption/ionization on porous silicon mass spectrometry for the detection of negative ions for fatty acids. Rapid Commun Mass Spectrom. 2006; 20(4):680-4. DOI: 10.1002/rcm.2363. View

16.

Wiklund S, Johansson E, Sjostrom L, Mellerowicz E, Edlund U, Shockcor J . Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models. Anal Chem. 2007; 80(1):115-22. DOI: 10.1021/ac0713510. View

17.

Finch-Savage W, Leubner-Metzger G . Seed dormancy and the control of germination. New Phytol. 2006; 171(3):501-23. DOI: 10.1111/j.1469-8137.2006.01787.x. View

18.

Gorzolka K, Kolling J, Nattkemper T, Niehaus K . Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging. PLoS One. 2016; 11(3):e0150208. PMC: 4777520. DOI: 10.1371/journal.pone.0150208. View

19.

Peukert M, Matros A, Lattanzio G, Kaspar S, Abadia J, Mock H . Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). New Phytol. 2011; 193(3):806-815. DOI: 10.1111/j.1469-8137.2011.03970.x. View

20.

Mohana Kumara P, Srimany A, Ravikanth G, Shaanker R, Pradeep T . Ambient ionization mass spectrometry imaging of rohitukine, a chromone anti-cancer alkaloid, during seed development in Dysoxylum binectariferum Hook.f (Meliaceae). Phytochemistry. 2015; 116:104-110. DOI: 10.1016/j.phytochem.2015.02.031. View