Design and Evaluation of the Immunogenicity and Efficacy of a Biomimetic Particulate Formulation of Viral Antigens

Overview

Journal Sci Rep

Specialty Science

Date 2017 Oct 25

PMID 29062078

Citations 12

Authors

Victor Riitho

Adam A Walters

Satyanarayana Somavarapu

Benjamin Lamp

Till Rumenapf

Thomas Krey

Felix A Rey

Ernesto Oviedo-Orta

Graham R Stewart

Nicolas Locker

Falko Steinbach

Simon P Graham

Affiliations

Soon will be listed here.

Abstract

Subunit viral vaccines are typically not as efficient as live attenuated or inactivated vaccines at inducing protective immune responses. This paper describes an alternative 'biomimetic' technology; whereby viral antigens were formulated around a polymeric shell in a rationally arranged fashion with a surface glycoprotein coated on to the surface and non-structural antigen and adjuvant encapsulated. We evaluated this model using BVDV E2 and NS3 proteins formulated in poly-(D, L-lactic-co-glycolic acid) (PLGA) nanoparticles adjuvanted with polyinosinic:polycytidylic acid (poly(I:C) as an adjuvant (Vaccine-NP). This Vaccine-NP was compared to ovalbumin and poly(I:C) formulated in a similar manner (Control-NP) and a commercial adjuvanted inactivated BVDV vaccine (IAV), all inoculated subcutaneously and boosted prior to BVDV-1 challenge. Significant virus-neutralizing activity, and E2 and NS3 specific antibodies were observed in both Vaccine-NP and IAV groups following the booster immunisation. IFN-γ responses were observed in ex vivo PBMC stimulated with E2 and NS3 proteins in both vaccinated groups. We observed that the protection afforded by the particulate vaccine was comparable to the licenced IAV formulation. In conclusion, the biomimetic particulates showed a promising immunogenicity and efficacy profile that may be improved by virtue of being a customisable mode of delivery.

Citing Articles

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