Prevalence of Selected Zoonotic and Vector-Borne Agents in Dogs and Cats on the Pine Ridge Reservation
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The prevalence of intestinal parasites and vector-borne agents of dogs and cats in the Pine Ridge Reservation, South Dakota were determined. Fecal samples (84 dogs, 9 cats) were examined by centrifugal floatation and by immunofluorescence assay (FA) for and . PCR was performed on [beta-giardin (bg), triose phosphate isomerase (tpi), glutamate dehydrogenase genes (gdh)] and [heat shock protein-70 gene (hsp)] FA positive samples. Cat sera ( = 32) were tested for antibodies against spp., , and FIV, and antigens of FeLV and . Dog sera ( = 82) were tested for antibodies against , , , and and antigen. Blood samples (92 dogs, 39 cats) were assessed by PCR for amplification of DNA of spp., spp., spp., haemoplasmas, and spp. (dogs only). The most significant results were spp. (32% by FA), spp. (17.8%) and spp. (7.1%). The isolates typed as the dog-specific assemblages C or D and four isolates typed as . Antibodies against were detected in 15% of the dogs. Antibodies against spp. and against were detected in 37.5% and 6% of the cats respectively. FeLV antigen was detected in 10% of the cats.
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