Impairment of Yeast Pre-mRNA Splicing by Potential Secondary Structure-forming Sequences Near the Conserved Branchpoint Sequence

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1988 Nov 25

PMID 2905037

Citations 13

Authors

H Halfter

D Gallwitz

Affiliations

Soon will be listed here.

Abstract

The absolutely conserved TACTAAC box within introns of RNA polymerase II-transcribed genes of the yeast Saccharomyces cerevisiae serves an indispensable role in lariat formation. We show in this report that rather short palindromic sequences inserted into the yeast actin gene intron immediately 3' to the TACTAAC box block the second but not the first splicing step. In contrast, a palindromic sequence inserted some 23 bp 3' of the TACTAAC box did not affect correct and efficient splicing. The data suggest that hairpin structures that might form adjacent to the branchsite sequence interfere with some necessary alteration of the spliceosome required for 3' intron cleavage and exon ligation.

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Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons.

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Impact on splicing in of random 50-base sequences inserted into an intron.

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Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons.

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Far upstream element-binding protein 1 and RNA secondary structure both mediate second-step splicing repression.

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Role of RNA structure in regulating pre-mRNA splicing.

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