Knockdown of Long Non-Coding RNA NEAT1 Inhibits Proliferation and Invasion and Induces Apoptosis of Osteosarcoma by Inhibiting MiR-194 Expression

Overview

Journal Yonsei Med J

Specialty General Medicine

Date 2017 Oct 20

PMID 29047232

Citations 17

Authors

Heping Wang

Yanzhang Yu

Shuxin Fan

Leifeng Luo

Affiliations

Soon will be listed here.

Abstract

Purpose: Long non-coding RNA (lncRNA) nuclear paraspeckle assembly transcript 1 (NEAT1) has been implicated as an oncogene in the development and progression of osteosarcoma. This study aims to explore the mechanism of NEAT1 in osteosarcoma.

Materials And Methods: Expressions of NEAT1 and miR-194 in osteosarcoma tissues and cells were detected by quantitative real-time PCR. The effects of NEAT1 knockdown or miR-194 overexpression on cell proliferation, invasion, and apoptosis were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay, transwell invasive assay, and flow cytometry analysis, respectively. Luciferase reporter assay was performed to observe the possible interaction between NEAT1 and miR-194.

Results: NEAT1 was upregulated and miR-194 was downregulated in osteosarcoma tissues and cells. Knockdown of NEAT1 or overexpression of miR-194 suppressed proliferation and invasion and induced apoptosis of osteosarcoma cells in vitro. Luciferase reporter assay validated that NEAT1 could interact with miR-194 and negatively modulated its expression. Furthermore, inhibition of miR-194 reversed the suppression of proliferation and invasion and the promotion of apoptosis induced by NEAT1 depletion in osteosarcoma cells.

Conclusion: Knockdown of NEAT1 suppressed proliferation and invasion and induced apoptosis in osteosarcoma cells by inhibiting miR-194 expression.

Citing Articles

Single-Cell Transcriptome Analysis Reveals Paraspeckles Expression in Osteosarcoma Tissues.

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Recent advances of NEAT1-miRNA interactions in cancer.

Zhou H, Wang Y, Liu Z, Zhang Z, Xiong L, Wen Y Acta Biochim Biophys Sin (Shanghai). 2022; 54(2):153-162.

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Zhang M, Guo J, Liu L, Huang M, Li Y, Bennett S Biochem Genet. 2021; 60(3):843-867.

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Chaudhary R Heliyon. 2021; 7(3):e06502.

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Wang L, Zhou J, Zhang Y, Hu T, Sun Y Int J Gen Med. 2021; 13:1685-1695.

PMID: 33408506 PMC: 7781107. DOI: 10.2147/IJGM.S274641.

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