Development of Adenosine Deaminase-Specific IgY Antibodies: Diagnostic and Inhibitory Application

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2017 Oct 19

PMID 29043661

Citations 6

Authors

Agnieszka Lupicka-Slowik

Mateusz Psurski

Renata Grzywa

Kamila Bobrek

Patrycja Smok

Maciej Walczak

Andrzej Gawel

Tadeusz Stefaniak

Jozef Oleksyszyn

Marcin Sienczyk

Affiliations

Soon will be listed here.

Abstract

Adenosine deaminase (ADA) is currently used as a diagnostic marker for tuberculous pleuritis. Although ADA has been suggested as a potential marker for several types of cancer, the importance of each of ADA isoforms as well as their levels and enzymatic activities in tumors need to be further investigated. Herein we developed avian immunoglobulin Y highly specific to human ADA via hens immunization with calf adenosine deaminase. The obtained antibodies were used for the development of a sensitive double-egg yolk immunoglobulin (IgY) sandwich ELISA assay with an ADA detection limit of 0.5 ng/ml and a linearity range of up to 10 ng/ml. Specific, affinity-purified IgYs were able to recognize human recombinant ADA and ADA present in human cancer cell lines. In addition, antigen-specific IgY antibodies were able to inhibit catalytic activity of calf ADA with an IC value of 47.48 nM. We showed that generated IgY antibodies may be useful for ADA detection, thus acting as a diagnostic agent in immunoenzymatic assays.

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