Corneal Cross-Linking Has No Effect on Matrix Metalloproteinase 9 and 13 Levels During Fungal Keratitis on the Early Stage

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2017 Oct 19

PMID 29043533

Citations 6

Authors

Ayse Kalkanci

Kamil Bilgihan

Huseyin Baran Ozdemir

Atiye Seda Yar Saglam

Funda Karakurt

Merve Erdogan

Affiliations

Soon will be listed here.

Abstract

The aim of our study was to investigate matrix metalloproteinases, MMP-9 and MMP-13 levels, in the rabbit model of Fusarium and Candida keratitis treated by corneal cross-linking (PACK-CXL). Rabbit corneas were inoculated with fungal inoculum for keratitis. Each group divided into four subgroups, including un-treated group, PACK-CXL group, voriconazole group and PACK-CXL plus voriconazole group. PACK-CXL was applied with 0.25% riboflavin in accelerated Dresden protocol, and 0.1% voriconazole drops were administered. All corneal buttons excised at tenth day after ophthalmological examination. Fungal cell counts and Scheiber scores were determined in all groups. Corneal tissue MMP mRNA levels were evaluated quantitative reverse transcriptase PCR. The difference in MMP-9 and MMP-13 levels at all groups was not statistically significant (p > 0.05). PACK-CXL with 0.25% riboflavin either alone or combined with antifungal drops was unable to provide decline in inflammatory findings in both macroscopic and microscopic levels similar to medical antifungal treatment.

Citing Articles

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Changes of matrix metalloproteinases in the stroma after corneal cross-linking in rabbits.

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The Role of Connexin-43 in the Inflammatory Process: A New Potential Therapy to Influence Keratitis.

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