Expression and Localization of Special AT-rich Sequence Binding Protein 2 in Murine Molar Development and the Pulp-dentin Complex of Human Healthy Teeth and Teeth with Pulpitis

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2017 Oct 19

PMID 29042940

Citations 6

Authors

Lina He

Huimei Liu

Lei Shi

Shuang Pan

Xu Yang

Lin Zhang

Yumei Niu

Affiliations

Soon will be listed here.

Abstract

Special AT-rich sequence binding protein 2 (SATB2) is a member of the special family of AT-rich binding transcription factors and has a critical role in osteoblast differentiation and craniofacial patterning. However, the expression and distribution of SATB2 in tooth development is largely unknown. The aim of the present study was to detect the expression and distribution of SATB2 during murine molar development and, in human healthy teeth and teeth with pulpitis using immunohistochemistry. Molars were obtained from Kunming mice at embryonic day (E) 13.5, E14.5, E16.5 and E18.5, and postnatal day (P) 1, P5 and P7. In addition, 20 human teeth (10 healthy and 10 teeth with pulpitis) were obtained from young adult patients (age, 24.90±1.65 years) who were scheduled for routine extraction. Immunohistochemical analyses were performed to detect the expression and distribution of SATB2. The present results revealed that SATB2 exhibits a spatiotemporal expression pattern in murine molar development and was expressed in odontoblasts, predentin, dental pulp cells and the blood vessels in human teeth. These findings suggested that SATB2 may have an important role in odontoblast differentiation and dentin matrix mineralization during tooth development.

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References

Tang W, Li Y, Osimiri L, Zhang C . Osteoblast-specific transcription factor Osterix (Osx) is an upstream regulator of Satb2 during bone formation. J Biol Chem. 2011; 286(38):32995-3002. PMC: 3190908. DOI: 10.1074/jbc.M111.244236. View

Sloan A, Rutherford R, Smith A . Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro. Arch Oral Biol. 2000; 45(2):173-7. DOI: 10.1016/s0003-9969(99)00131-4. View

Komori T . Regulation of bone development and extracellular matrix protein genes by RUNX2. Cell Tissue Res. 2009; 339(1):189-95. DOI: 10.1007/s00441-009-0832-8. View

Feng J, McDaniel J, Chuang H, Huang O, Rakian A, Xu X . Binding of amelogenin to MMP-9 and their co-expression in developing mouse teeth. J Mol Histol. 2012; 43(5):473-85. PMC: 3460178. DOI: 10.1007/s10735-012-9423-1. View

Simon S, Smith A, Lumley P, Berdal A, Smith G, Finney S . Molecular characterization of young and mature odontoblasts. Bone. 2009; 45(4):693-703. DOI: 10.1016/j.bone.2009.06.018. View

Bleicher F, Couble M, Farges J, Couble P, Magloire H . Sequential expression of matrix protein genes in developing rat teeth. Matrix Biol. 1999; 18(2):133-43. DOI: 10.1016/s0945-053x(99)00007-4. View

Yang G, Li X, Yuan G, Liu P, Fan M . The effects of osterix on the proliferation and odontoblastic differentiation of human dental papilla cells. J Endod. 2014; 40(11):1771-7. DOI: 10.1016/j.joen.2014.04.012. View

Zhang P, Men J, Fu Y, Shan T, Ye J, Wu Y . Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones. Cell Tissue Res. 2012; 350(3):425-37. DOI: 10.1007/s00441-012-1487-4. View

Wei J, Shi Y, Zheng L, Zhou B, Inose H, Wang J . miR-34s inhibit osteoblast proliferation and differentiation in the mouse by targeting SATB2. J Cell Biol. 2012; 197(4):509-21. PMC: 3352956. DOI: 10.1083/jcb.201201057. View

10.

Britanova O, Akopov S, Lukyanov S, Gruss P, Tarabykin V . Novel transcription factor Satb2 interacts with matrix attachment region DNA elements in a tissue-specific manner and demonstrates cell-type-dependent expression in the developing mouse CNS. Eur J Neurosci. 2005; 21(3):658-68. DOI: 10.1111/j.1460-9568.2005.03897.x. View

11.

Smith A, Murray P, Sloan A, Matthews J, Zhao S . Trans-dentinal stimulation of tertiary dentinogenesis. Adv Dent Res. 2003; 15:51-4. DOI: 10.1177/08959374010150011301. View

12.

Fitzpatrick D, Carr I, McLaren L, Leek J, Wightman P, Williamson K . Identification of SATB2 as the cleft palate gene on 2q32-q33. Hum Mol Genet. 2003; 12(19):2491-501. DOI: 10.1093/hmg/ddg248. View

13.

Chen S, Gluhak-Heinrich J, Wang Y, Wu Y, Chuang H, Chen L . Runx2, osx, and dspp in tooth development. J Dent Res. 2009; 88(10):904-9. PMC: 3045537. DOI: 10.1177/0022034509342873. View

14.

Chen Z, Couble M, Mouterfi N, Magloire H, Chen Z, Bleicher F . Spatial and temporal expression of KLF4 and KLF5 during murine tooth development. Arch Oral Biol. 2009; 54(5):403-11. DOI: 10.1016/j.archoralbio.2009.02.003. View

15.

Fang P, Wang X, Zhang L, Yuan G, Chen Z, Zhang Q . Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development. J Mol Histol. 2010; 41(4-5):199-203. DOI: 10.1007/s10735-010-9279-1. View

16.

Hu N, Feng C, Jiang Y, Miao Q, Liu H . Regulative Effect of Mir-205 on Osteogenic Differentiation of Bone Mesenchymal Stem Cells (BMSCs): Possible Role of SATB2/Runx2 and ERK/MAPK Pathway. Int J Mol Sci. 2015; 16(5):10491-506. PMC: 4463658. DOI: 10.3390/ijms160510491. View

17.

Li S, Kong H, Yao N, Yu Q, Wang P, Lin Y . The role of runt-related transcription factor 2 (Runx2) in the late stage of odontoblast differentiation and dentin formation. Biochem Biophys Res Commun. 2011; 410(3):698-704. DOI: 10.1016/j.bbrc.2011.06.065. View

18.

Aberg T, Wang X, Kim J, Yamashiro T, Bei M, Rice R . Runx2 mediates FGF signaling from epithelium to mesenchyme during tooth morphogenesis. Dev Biol. 2004; 270(1):76-93. DOI: 10.1016/j.ydbio.2004.02.012. View

19.

Kim I, Jeong S, Kim S, Jung J, Park Y, Kim S . Special AT-rich sequence-binding protein 2 and its related genes play key roles in the differentiation of MC3T3-E1 osteoblast like cells. Biochem Biophys Res Commun. 2011; 417(2):697-703. DOI: 10.1016/j.bbrc.2011.11.157. View

20.

Viriot L, Peterkova R, Vonesch J, Peterka M, RUCH J, Lesot H . Mouse molar morphogenesis revisited by three-dimensional reconstruction. III. Spatial distribution of mitoses and apoptoses up to bell-staged first lower molar teeth. Int J Dev Biol. 1998; 41(5):679-90. View